Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 975615..976231 Replicon chromosome
Accession NZ_CP052858
Organism Pseudomonas sp. ADAK20

Toxin (Protein)


Gene name hicA Uniprot ID A0A1S1UW78
Locus tag HKK56_RS04480 Protein ID WP_029292612.1
Coordinates 975615..975803 (+) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7Y1DEL1
Locus tag HKK56_RS04485 Protein ID WP_029292614.1
Coordinates 975827..976231 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKK56_RS04450 971235..971771 + 537 WP_029292599.1 DsbE family thiol:disulfide interchange protein -
HKK56_RS04455 971768..972241 + 474 WP_029292601.1 cytochrome c-type biogenesis protein CcmH -
HKK56_RS04460 972234..973427 + 1194 WP_029292603.1 c-type cytochrome biogenesis protein CcmI -
HKK56_RS04465 973444..973839 + 396 WP_029292605.1 hypothetical protein -
HKK56_RS04470 974187..974753 + 567 WP_169391168.1 phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN -
HKK56_RS04475 974744..975493 + 750 WP_169389700.1 phosphonate metabolism protein PhnP -
HKK56_RS04480 975615..975803 + 189 WP_029292612.1 type II toxin-antitoxin system HicA family toxin Toxin
HKK56_RS04485 975827..976231 + 405 WP_029292614.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HKK56_RS04490 976377..977573 - 1197 WP_178121969.1 MFS transporter -
HKK56_RS04495 977815..978819 - 1005 WP_029292620.1 sulfate ABC transporter substrate-binding protein -
HKK56_RS04500 978913..979737 - 825 WP_029292622.1 ion transporter -
HKK56_RS04505 979769..980644 - 876 WP_169389699.1 urea transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7120.29 Da        Isoelectric Point: 11.5133

>T155644 WP_029292612.1 NZ_CP052858:975615-975803 [Pseudomonas sp. ADAK20]
VQSRLLIKELQAAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRQRAGLFSF

Download         Length: 189 bp

>T155644 NZ_CP068810:c830023-829646 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14742.74 Da        Isoelectric Point: 4.6115

>AT155644 WP_029292614.1 NZ_CP052858:975827-976231 [Pseudomonas sp. ADAK20]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEMASEGRAIPMPTSVAAHHGNEEYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Download         Length: 405 bp

>AT155644 NZ_CP068810:c830481-830113 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S1UW78

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y1DEL1

References