Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2865377..2866019 Replicon chromosome
Accession NZ_CP051858
Organism Bacillus thuringiensis serovar israelensis strain BGSC 4Q7rifR

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag HIS92_RS14750 Protein ID WP_000635965.1
Coordinates 2865669..2866019 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag HIS92_RS14745 Protein ID WP_000004570.1
Coordinates 2865377..2865664 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HIS92_RS14720 2860694..2861656 + 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -
HIS92_RS14725 2861649..2862221 - 573 WP_000908528.1 rhomboid family intramembrane serine protease -
HIS92_RS14730 2862315..2862674 + 360 WP_000583420.1 holo-ACP synthase -
HIS92_RS14735 2862831..2863781 + 951 WP_003309006.1 outer membrane lipoprotein carrier protein LolA -
HIS92_RS14740 2863899..2865068 + 1170 WP_000390594.1 alanine racemase -
HIS92_RS14745 2865377..2865664 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
HIS92_RS14750 2865669..2866019 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HIS92_RS14755 2866087..2868255 + 2169 WP_000426211.1 RNA-binding transcriptional accessory protein -
HIS92_RS14760 2868313..2868429 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
HIS92_RS14765 2868625..2869083 + 459 WP_000343552.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T155359 WP_000635965.1 NZ_CP051858:2865669-2866019 [Bacillus thuringiensis serovar israelensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T155359 NZ_CP068799:1984979-1985233 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT155359 WP_000004570.1 NZ_CP051858:2865377-2865664 [Bacillus thuringiensis serovar israelensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT155359 NZ_CP068799:1984731-1984982 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References