Detailed information of TA system TA155355

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4820971..4821806
• Replicon: chromosome
• Accession: NZ_CP051849
• Organism: Escherichia coli strain SCU-101

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: HHJ20_RS23410
• Protein ID: WP_001094445.1
• Coordinates: 4821429..4821806 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HHJ20_RS23405
• Protein ID: WP_024174331.1
• Coordinates: 4820971..4821339 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ20_RS23375 (4817727)	4817727..4818182	+	456	WP_000581502.1	IrmA family protein	-
HHJ20_RS23380 (4818464)	4818464..4819285	+	822	WP_021556204.1	DUF932 domain-containing protein	-
HHJ20_RS23385 (4819285)	4819285..4819530	+	246	WP_001164966.1	hypothetical protein	-
HHJ20_RS23390 (4819624)	4819624..4820097	+	474	WP_001313575.1	antirestriction protein	-
HHJ20_RS23395 (4820113)	4820113..4820589	+	477	WP_001186170.1	RadC family protein	-
HHJ20_RS23400 (4820676)	4820676..4820897	+	222	WP_000692346.1	DUF987 domain-containing protein	-
HHJ20_RS23405 (4820971)	4820971..4821339	+	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ20_RS23410 (4821429)	4821429..4821806	+	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
HHJ20_RS23415 (4821803)	4821803..4822291	+	489	WP_001534777.1	DUF5983 family protein	-
HHJ20_RS23420 (4822303)	4822303..4822500	+	198	WP_000838536.1	DUF957 domain-containing protein	-
HHJ20_RS23425 (4822585)	4822585..4823427	+	843	WP_001280501.1	DUF4942 domain-containing protein	-
HHJ20_RS23430 (4824176)	4824176..4825714	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T155355 WP_001094445.1 NZ_CP051849:4821429-4821806 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T155355 NZ_CP068799:c830872-830498 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT155355 WP_024174331.1 NZ_CP051849:4820971-4821339 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT155355 NZ_CP068799:c831330-830962 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACAGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454A7U6