Detailed information of TA system TA155331

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 885368..886202
• Replicon: chromosome
• Accession: NZ_CP051849
• Organism: Escherichia coli strain SCU-101

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H2VDB0
• Locus tag: HHJ20_RS04275
• Protein ID: WP_000854688.1
• Coordinates: 885368..885745 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0H2VAY1
• Locus tag: HHJ20_RS04280
• Protein ID: WP_001285596.1
• Coordinates: 885822..886202 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ20_RS04245 (881760)	881760..881930	-	171	Protein_845	IS110 family transposase	-
HHJ20_RS04250 (882347)	882347..883281	-	935	Protein_846	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HHJ20_RS04255 (883274)	883274..883669	-	396	WP_000208383.1	DUF6088 family protein	-
HHJ20_RS04260 (883738)	883738..884583	-	846	WP_001280441.1	DUF4942 domain-containing protein	-
HHJ20_RS04265 (884668)	884668..884865	-	198	WP_000839260.1	DUF957 domain-containing protein	-
HHJ20_RS04270 (884882)	884882..885371	-	490	Protein_850	DUF5983 family protein	-
HHJ20_RS04275 (885368)	885368..885745	-	378	WP_000854688.1	TA system toxin CbtA family protein	Toxin
HHJ20_RS04280 (885822)	885822..886202	-	381	WP_001285596.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ20_RS04285 (886252)	886252..886896	-	645	WP_000094917.1	hypothetical protein	-
HHJ20_RS04290 (886915)	886915..887136	-	222	WP_000692329.1	DUF987 domain-containing protein	-
HHJ20_RS04295 (887199)	887199..887675	-	477	WP_001186726.1	RadC family protein	-
HHJ20_RS04300 (887691)	887691..888176	-	486	WP_000849565.1	antirestriction protein	-
HHJ20_RS04305 (888231)	888231..889052	-	822	WP_166726888.1	DUF932 domain-containing protein	-
HHJ20_RS26085 (889231)	889231..889320	-	90	WP_230607454.1	DUF905 family protein	-
HHJ20_RS04315 (889463)	889463..889918	-	456	WP_000581502.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsM / kpsT / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF	863749..888176	24427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14014.01 Da
• Isoelectric Point: 8.5221

>T155331 WP_000854688.1 NZ_CP051849:c885745-885368 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T155331 NZ_CP068796:c4686487-4686314 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13897.70 Da
• Isoelectric Point: 4.7959

>AT155331 WP_001285596.1 NZ_CP051849:c886202-885822 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCAVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT155331 NZ_CP068796:c4686811-4686515 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0H2VDB0

Antitoxin

• AlphaFold DB: A0A0H2VAY1