Detailed information of TA system TA155247

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36872..37136
• Replicon: plasmid pSCU-484-1
• Accession: NZ_CP051745
• Organism: Escherichia coli strain SCU-484

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: HHJ53_RS24815
• Protein ID: WP_001331364.1
• Coordinates: 36984..37136 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	36872..36929 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ53_RS24800	32914..33984	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
HHJ53_RS24805	34003..35211	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
HHJ53_RS24810	35429..36415	-	987	WP_001257838.1	hypothetical protein	-
-	36576..36631	-	56	NuclAT_1	-	-
-	36576..36631	-	56	NuclAT_1	-	-
-	36576..36631	-	56	NuclAT_1	-	-
-	36576..36631	-	56	NuclAT_1	-	-
-	36872..36929	-	58	NuclAT_0	-	Antitoxin
-	36872..36929	-	58	NuclAT_0	-	Antitoxin
-	36872..36929	-	58	NuclAT_0	-	Antitoxin
-	36872..36929	-	58	NuclAT_0	-	Antitoxin
HHJ53_RS24815	36984..37136	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
HHJ53_RS24820	37208..37459	-	252	WP_001291964.1	hypothetical protein	-
HHJ53_RS24825	37759..38055	+	297	WP_001275298.1	hypothetical protein	-
HHJ53_RS24830	38120..38296	-	177	WP_001054900.1	hypothetical protein	-
HHJ53_RS24835	38628..38837	+	210	WP_000062602.1	HEAT repeat domain-containing protein	-
HHJ53_RS24840	38909..39571	-	663	WP_012783935.1	plasmid IncI1-type surface exclusion protein ExcA	-
HHJ53_RS24845	39642..41810	-	2169	WP_023518847.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..80332	80332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T155247 WP_001331364.1 NZ_CP051745:36984-37136 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T155247 NZ_CP068710:c2569710-2568805 [Burkholderia thailandensis]
ATGAGCGCAATCTCCACGACGACTACCGATGCGAGCGCGACGCCGTTCGCCGCGCTGCTCGAACGCTTTGCCGAGCGGCG
CGCGGCCGCAGGCGGCGCGCGTACGATCGGCTATCGCGAAGCGGGCGCAGCGTACGCAGCGCATGCCGGGCGCGATCCCG
TCGTGTTGCTGCACGGGATCGGATCGGGTGCGGCGTCGTGGGTGCGTCAACTCGATGCGCTCGGCGCGACGCGTCGCGCG
CTCGCGTGGGACGCGCCGGGCTATGGCGTCTCGTCGCGCGTCGCGGCCGATTCGCCTGTCGCATCCGACTACGCGGCGTC
GCTCGCCGCGTGGCTCGACGCGCTGCGGATCGAACGCTGCGTGCTCGTCGGCCATTCGCTCGGCGCGATCGTCGCCGGCG
CGTTCGCGCGGCAAGCGGGCGAGCGTCTTTCGGGATTGCTGCTGATTTCTCCGGCGGGCGGCTACGGCGGCGCACCCGCC
GACGTGCGGGCCGAGCGGCGCGACAGCCGCCTCGCGCTGCTCGCGTCGCTCGGCCCGCAAGGGCTCGCCGCCGAGCGCAG
CGCAAACATGCTGTCCGCGCAGGCGAGCGACGCCGCGCGCGCGTGGGTGCGCTGGAACATGGCGCGCATCGTGCCCGCCG
GCTATGCGCAGGCGACGCACCTGCTCGCGAACGCGGACCTCGCGTCCGATCTCGCGCATTATCGCGGCCGCGTCGCGGTC
GCGGTCGGCGCCGACGATACGATCACGCCGCCCGCCGCGTGCGAGCGGATCGCGCGGGCAGCGGGCGCCGGGTTGCAGGT
GATCCCGCGCGCGGGCCATGCCGGCTATGTCGAAGCGCCGGATGCATACAACGCGTTGATCGACGCATTTTGCCGTCAAT
GCGACGGATCGCGAGGTGCGGCATGA

Antitoxin

Nucleotide

• Length: 58 bp

>AT155247 NZ_CP051745:c36929-36872 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin