Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2606776..2607338 Replicon chromosome
Accession NZ_CP051738
Organism Escherichia coli strain SCU-105

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag HHJ23_RS12675 Protein ID WP_000605676.1
Coordinates 2606776..2607054 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HHJ23_RS12680 Protein ID WP_000781370.1
Coordinates 2607054..2607338 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ23_RS12650 2602362..2602793 - 432 WP_000152305.1 peroxiredoxin OsmC -
HHJ23_RS12655 2603138..2603353 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HHJ23_RS12660 2603455..2603592 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HHJ23_RS12665 2603749..2605446 + 1698 WP_000433464.1 malate dehydrogenase -
HHJ23_RS12670 2605580..2606590 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
HHJ23_RS12675 2606776..2607054 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHJ23_RS12680 2607054..2607338 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HHJ23_RS12685 2607566..2608219 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HHJ23_RS12690 2608212..2609096 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
HHJ23_RS12695 2609109..2612156 - 3048 WP_158104837.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T155200 WP_000605676.1 NZ_CP051738:2606776-2607054 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T155200 NZ_CP068696:1321709-1322107 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTTTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT155200 WP_000781370.1 NZ_CP051738:2607054-2607338 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT155200 NZ_CP068696:1321482-1321709 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGCAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References