Detailed information of TA system TA155116

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65373..65626
• Replicon: plasmid pSCU-111-2
• Accession: NZ_CP051729
• Organism: Escherichia coli strain SCU-111

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HHJ28_RS24115
• Protein ID: WP_001312851.1
• Coordinates: 65477..65626 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	65373..65429 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ28_RS24085	61899..62645	+	747	WP_169062165.1	conjugal transfer pilus acetylase TraX	-
HHJ28_RS24090	62700..63260	+	561	WP_040084627.1	fertility inhibition protein FinO	-
HHJ28_RS24095	63396..63608	+	213	WP_040084624.1	hypothetical protein	-
HHJ28_RS24100	63854..64315	+	462	WP_040084621.1	thermonuclease family protein	-
HHJ28_RS24105	64384..64569	+	186	WP_040084637.1	hypothetical protein	-
HHJ28_RS24110	64608..65198	+	591	WP_040084619.1	DUF2726 domain-containing protein	-
-	65373..65429	-	57	NuclAT_0	-	Antitoxin
-	65373..65429	-	57	NuclAT_0	-	Antitoxin
-	65373..65429	-	57	NuclAT_0	-	Antitoxin
-	65373..65429	-	57	NuclAT_0	-	Antitoxin
HHJ28_RS24115	65477..65626	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
HHJ28_RS24120	65910..66167	+	258	WP_000083826.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66467	66467

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T155116 WP_001312851.1 NZ_CP051729:65477-65626 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T155116 NZ_CP068678:3492409-3492699 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT155116 NZ_CP051729:c65429-65373 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin