Detailed information of TA system TA155110

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 139747..140173
• Replicon: plasmid pSCU-111-1
• Accession: NZ_CP051728
• Organism: Escherichia coli strain SCU-111

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HHJ28_RS23525
• Protein ID: WP_001312861.1
• Coordinates: 139747..139905 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	139949..140173 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ28_RS23495	135564..135947	-	384	WP_169062112.1	relaxosome protein TraM	-
HHJ28_RS23500	136268..136870	+	603	WP_169062113.1	transglycosylase SLT domain-containing protein	-
HHJ28_RS23505	137167..137988	-	822	WP_169062146.1	DUF945 domain-containing protein	-
HHJ28_RS23510	138107..138394	-	288	WP_000107542.1	hypothetical protein	-
HHJ28_RS23515	138550..138852	-	303	WP_169062114.1	hypothetical protein	-
HHJ28_RS24330	138876..139082	-	207	WP_000275848.1	hypothetical protein	-
HHJ28_RS23525	139747..139905	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	139949..140173	-	225	NuclAT_0	-	Antitoxin
-	139949..140173	-	225	NuclAT_0	-	Antitoxin
-	139949..140173	-	225	NuclAT_0	-	Antitoxin
-	139949..140173	-	225	NuclAT_0	-	Antitoxin
HHJ28_RS23530	139985..140173	+	189	WP_169062067.1	protein sok	-
HHJ28_RS23535	140142..140424	-	283	Protein_150	plasmid SOS inhibition protein A	-
HHJ28_RS23540	140491..141273	-	783	WP_097748551.1	IS21-like element IS100kyp family helper ATPase IstB	-
HHJ28_RS23545	141270..142256	-	987	WP_169062115.1	IS21 family transposase	-
HHJ28_RS23550	142238..142864	+	627	Protein_153	hypothetical protein	-
HHJ28_RS23555	143184..143663	+	480	WP_024130283.1	GNAT family N-acetyltransferase	-
HHJ28_RS23560	144119..145140	+	1022	Protein_155	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	acrB / pet / etpB / etpB / cdtC / cdtB / cdtA / etpA / etpB	1..189717	189717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T155110 WP_001312861.1 NZ_CP051728:c139905-139747 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T155110 NZ_CP068651:22745-23014 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTACTGTCGTGGACTGATGACGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAACAAACTCATCAATGATATTAAGCGTTCCTCATTTGAGGGCATCGGTAAACCAGAGCCATTAA
AAGAGAACTTATCTGGCTTTTGGTCTCGCCGTATTGATGACACTAATAGGCTTGTTTACGCAGTCGATGACCAAGCTATA
ACGATAATTTCGTGTCGTTATCACTACTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT155110 NZ_CP051728:c140173-139949 [Escherichia coli]
TCACACGGATTTCCCGTGAACAGCCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin