Detailed information of TA system TA154996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2382608..2383246
• Replicon: chromosome
• Accession: NZ_CP051716
• Organism: Escherichia coli strain SCU-118

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: HHJ32_RS11455
• Protein ID: WP_001447010.1
• Coordinates: 2383070..2383246 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HHJ32_RS11450
• Protein ID: WP_001270286.1
• Coordinates: 2382608..2383024 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ32_RS11430	2377760..2378701	-	942	WP_001251304.1	ABC transporter permease	-
HHJ32_RS11435	2378702..2379715	-	1014	WP_047609422.1	ABC transporter ATP-binding protein	-
HHJ32_RS11440	2379733..2380878	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HHJ32_RS11445	2381123..2382529	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HHJ32_RS11450	2382608..2383024	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HHJ32_RS11455	2383070..2383246	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HHJ32_RS11460	2383468..2383698	+	231	WP_000494244.1	YncJ family protein	-
HHJ32_RS11465	2383790..2385751	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HHJ32_RS11470	2385824..2386360	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HHJ32_RS11475	2386413..2387627	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T154996 WP_001447010.1 NZ_CP051716:c2383246-2383070 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T154996 NZ_CP068591:334104-334415 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT154996 WP_001270286.1 NZ_CP051716:c2383024-2382608 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT154996 NZ_CP068591:334416-334706 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8