Detailed information of TA system TA154931

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 816844..817675
• Replicon: chromosome
• Accession: NZ_CP051711
• Organism: Escherichia coli strain SCU-123

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: HHJ36_RS03865
• Protein ID: WP_000854814.1
• Coordinates: 817301..817675 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1PWQ3
• Locus tag: HHJ36_RS03860
• Protein ID: WP_001285586.1
• Coordinates: 816844..817212 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ36_RS03825	812061..813131	+	1071	WP_000102631.1	patatin-like phospholipase family protein	-
HHJ36_RS03830	813267..813944	+	678	WP_001362823.1	hypothetical protein	-
HHJ36_RS03835	813960..814370	+	411	WP_000846704.1	hypothetical protein	-
HHJ36_RS03840	814591..815412	+	822	WP_001234569.1	DUF945 domain-containing protein	-
HHJ36_RS03845	815503..815988	+	486	WP_000213703.1	antirestriction protein	-
HHJ36_RS03850	816004..816480	+	477	WP_001351157.1	RadC family protein	-
HHJ36_RS03855	816549..816770	+	222	WP_000692323.1	DUF987 domain-containing protein	-
HHJ36_RS03860	816844..817212	+	369	WP_001285586.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HHJ36_RS03865	817301..817675	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HHJ36_RS03870	817672..817866	+	195	WP_000988601.1	hypothetical protein	-
HHJ36_RS03875	817879..817992	+	114	WP_001161660.1	DUF957 domain-containing protein	-
HHJ36_RS03880	818281..818427	-	147	Protein_762	transposase domain-containing protein	-
HHJ36_RS03885	818481..818663	+	183	WP_001016348.1	hypothetical protein	-
HHJ36_RS03890	818764..819093	-	330	WP_000450409.1	DUF496 family protein	-
HHJ36_RS03895	819265..820323	-	1059	WP_001200890.1	FUSC family protein	-
HHJ36_RS03900	820521..820994	-	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
HHJ36_RS03905	821113..822279	-	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T154931 WP_000854814.1 NZ_CP051711:817301-817675 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T154931 NZ_CP068434:c1460521-1460243 [Cupriavidus necator]
ATGATCACTTCGTTCAACTGCCGCGACACGGCACTGCTCTTCGCAGGCACGCGGATCGCCCGGTTCGTCAACATAGAATC
TGTTGCCATGCGCAAGCTCCAGCAGTTGCACGCGGCCGGCGACCTCAGCTTTCTGCGAGTCCCGCCGGGTAACAGACTGG
AAGCGCTGCAAGGGGACCGGGCTGGTCAGCACAGCATCCGCTTCAACGACCAATGGCGAATCTGCTTTTGCTTCGCCAAT
GGCCATGCAACCGACGTCGAGATCGTCGACTACCACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13563.41 Da
• Isoelectric Point: 5.0468

>AT154931 WP_001285586.1 NZ_CP051711:816844-817212 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT154931 NZ_CP068434:c1460230-1459928 [Cupriavidus necator]
ATGGCACGCGAAGTACCCCTGGCCACTCCGGGAGAAATCCTTCAACTGGACTGGCTCGACCCGCTTGGCATTTCTCAGTA
CGCCCTGGCCAAGGCCATCGACGTACCGCCTCGCCGTATCAATGAAATTGTGAAGGGTGGCCGCGCCATAACGGCGGACA
CCGCTGTACGCCTTGGTGCGTTCTTCGGCACCGATCCCCAGGGTTGGATGAACCTGCAGACGCACTTCGACACCGAAATG
GCAAAGGAGAAGATCGGCGAGAAGAAGCTGGAGTCGATCAGGCAGCATGCCGTGCTGGCATGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: S1PWQ3