Detailed information of TA system TA154919

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 71864..72117
• Replicon: plasmid pSCU-124-2
• Accession: NZ_CP051708
• Organism: Escherichia coli strain SCU-124

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: HHJ37_RS23900
• Protein ID: WP_001336447.1
• Coordinates: 71968..72117 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	71864..71920 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ37_RS23870	67264..67821	+	558	WP_169063479.1	fertility inhibition protein FinO	-
HHJ37_RS23875	67973..68176	+	204	WP_001336517.1	hypothetical protein	-
HHJ37_RS23880	68860..70073	+	1214	WP_162901046.1	IS3 family transposase	-
HHJ37_RS23885	70231..70692	+	462	WP_000760080.1	thermonuclease family protein	-
HHJ37_RS24075	70795..71179	+	385	Protein_84	hypothetical protein	-
HHJ37_RS23895	71332..71769	+	438	WP_000872609.1	hypothetical protein	-
-	71864..71920	-	57	NuclAT_1	-	Antitoxin
-	71864..71920	-	57	NuclAT_1	-	Antitoxin
-	71864..71920	-	57	NuclAT_1	-	Antitoxin
-	71864..71920	-	57	NuclAT_1	-	Antitoxin
HHJ37_RS23900	71968..72117	+	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
HHJ37_RS23905	72402..72659	+	258	WP_000084404.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..72961	72961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T154919 WP_001336447.1 NZ_CP051708:71968-72117 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T154919 NZ_CP068394:4698853-4699026 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 57 bp

>AT154919 NZ_CP051708:c71920-71864 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin