Detailed information of TA system TA154915

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30296..30566
• Replicon: plasmid pSCU-124-2
• Accession: NZ_CP051708
• Organism: Escherichia coli strain SCU-124

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HHJ37_RS23655
• Protein ID: WP_001312861.1
• Coordinates: 30408..30566 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30296..30359 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ37_RS23620	25314..25766	-	453	WP_024185774.1	hypothetical protein	-
HHJ37_RS23625	26068..26595	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HHJ37_RS23630	26653..26886	+	234	WP_033551487.1	DUF905 family protein	-
HHJ37_RS23635	26945..28909	+	1965	WP_169063476.1	ParB/RepB/Spo0J family partition protein	-
HHJ37_RS23640	28978..29412	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HHJ37_RS23645	29409..30128	+	720	WP_113365649.1	plasmid SOS inhibition protein A	-
-	30140..30364	+	225	NuclAT_0	-	-
-	30140..30364	+	225	NuclAT_0	-	-
-	30140..30364	+	225	NuclAT_0	-	-
-	30140..30364	+	225	NuclAT_0	-	-
HHJ37_RS23650	30149..30328	-	180	WP_001309233.1	hypothetical protein	-
-	30296..30359	-	64	-	-	Antitoxin
HHJ37_RS23655	30408..30566	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HHJ37_RS23660	30804..31139	-	336	WP_032195784.1	hypothetical protein	-
HHJ37_RS23670	31482..31778	+	297	WP_001272251.1	hypothetical protein	-
HHJ37_RS23675	31889..32710	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HHJ37_RS23680	33007..33609	-	603	Protein_42	transglycosylase SLT domain-containing protein	-
HHJ37_RS23685	33930..34313	+	384	WP_001151538.1	relaxosome protein TraM	-
HHJ37_RS23690	34500..35189	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..72961	72961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T154915 WP_001312861.1 NZ_CP051708:30408-30566 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T154915 NZ_CP068394:3371723-3372097 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Nucleotide

• Length: 64 bp

>AT154915 NZ_CP051708:c30359-30296 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin