Detailed information of TA system TA154864

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89498..89880
• Replicon: plasmid pSCU-152-1
• Accession: NZ_CP051699
• Organism: Escherichia coli strain SCU-152

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: HHJ40_RS22985
• Protein ID: WP_000775238.1
• Coordinates: 89498..89659 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	89705..89880 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ40_RS22945	84573..84800	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
HHJ40_RS22950	84894..85580	-	687	WP_000332492.1	PAS domain-containing protein	-
HHJ40_RS22955	85770..86153	-	384	WP_000124981.1	relaxosome protein TraM	-
HHJ40_RS22960	86487..87077	+	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-
HHJ40_RS22965	87373..88194	-	822	WP_143368504.1	DUF945 domain-containing protein	-
HHJ40_RS22970	88313..88600	-	288	WP_096932983.1	hypothetical protein	-
HHJ40_RS23325	88901..89261	+	361	Protein_76	hypothetical protein	-
HHJ40_RS22985	89498..89659	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	89705..89880	+	176	-	-	Antitoxin
-	89705..89900	-	196	NuclAT_0	-	-
-	89705..89900	-	196	NuclAT_0	-	-
-	89705..89900	-	196	NuclAT_0	-	-
-	89705..89900	-	196	NuclAT_0	-	-
HHJ40_RS22990	89878..90080	+	203	Protein_78	transposase	-
HHJ40_RS22995	90099..90392	-	294	WP_096932981.1	helix-turn-helix transcriptional regulator	-
HHJ40_RS23000	90501..91280	-	780	WP_096933013.1	EAL domain-containing protein	-
HHJ40_RS23005	91419..92231	-	813	Protein_81	hypothetical protein	-
HHJ40_RS23010	92459..92989	-	531	WP_096932979.1	fimbrial protein	-
HHJ40_RS23015	92976..93662	-	687	WP_096932978.1	fimbrial chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic	1..117034	117034

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T154864 WP_000775238.1 NZ_CP051699:c89659-89498 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T154864 NZ_CP068287:2288717-2289202 [Enterobacter hormaechei]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTACTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTCGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCG
CAGTAG

Antitoxin

Nucleotide

• Length: 176 bp

>AT154864 NZ_CP051699:89705-89880 [Escherichia coli]
GTGGACTAGACATGCAGAGGCCTCGTGGGTTAATGAAAATTAACTACGGGGCTTTTGTCCTTCTGCCACACAACACGGTA
ACAAACCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGCTGACCACA
CTCACTTTTCCTGAAA

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin