Detailed information of TA system TA154834

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25429..25698
• Replicon: plasmid pSCU-313-2
• Accession: NZ_CP051696
• Organism: Escherichia coli strain SCU-313

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HHJ46_RS25185
• Protein ID: WP_001312861.1
• Coordinates: 25540..25698 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	25429..25494 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ46_RS25150	20458..20901	-	444	Protein_28	hypothetical protein	-
HHJ46_RS25155	21198..21725	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HHJ46_RS25160	21783..22016	+	234	WP_000006003.1	DUF905 family protein	-
HHJ46_RS25165	22077..24041	+	1965	WP_169062239.1	ParB/RepB/Spo0J family partition protein	-
HHJ46_RS25170	24110..24544	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HHJ46_RS25175	24541..25260	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	25272..25496	+	225	NuclAT_0	-	-
-	25272..25496	+	225	NuclAT_0	-	-
-	25272..25496	+	225	NuclAT_0	-	-
-	25272..25496	+	225	NuclAT_0	-	-
HHJ46_RS25180	25281..25460	-	180	WP_001309233.1	hypothetical protein	-
-	25429..25494	+	66	-	-	Antitoxin
HHJ46_RS25185	25540..25698	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HHJ46_RS25190	25936..26314	-	379	Protein_36	hypothetical protein	-
HHJ46_RS25195	26620..26916	+	297	WP_001272251.1	hypothetical protein	-
HHJ46_RS25200	27027..27848	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HHJ46_RS25205	28145..28792	-	648	WP_128438058.1	transglycosylase SLT domain-containing protein	-
HHJ46_RS25210	29068..29451	+	384	WP_001151529.1	relaxosome protein TraM	-
HHJ46_RS25215	29643..30290	+	648	WP_169062240.1	transcriptional regulator TraJ family protein	-
HHJ46_RS25220	30398..30637	+	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67142	67142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T154834 WP_001312861.1 NZ_CP051696:25540-25698 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T154834 NZ_CP068283:c1626991-1625669 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT154834 NZ_CP051696:25429-25494 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin