Detailed information of TA system TA154824

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 4348591..4349003
• Replicon: chromosome
• Accession: NZ_CP051694
• Organism: Escherichia coli strain SCU-313

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: HHJ46_RS20945
• Protein ID: WP_000132601.1
• Coordinates: 4348662..4349003 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	4348591..4348667 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ46_RS20935	4345454..4347043	+	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-
HHJ46_RS20940	4347040..4348434	+	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
-	4348591..4348667	-	77	NuclAT_10	-	Antitoxin
-	4348591..4348667	-	77	NuclAT_10	-	Antitoxin
-	4348591..4348667	-	77	NuclAT_10	-	Antitoxin
-	4348591..4348667	-	77	NuclAT_10	-	Antitoxin
-	4348591..4348667	-	77	NuclAT_11	-	Antitoxin
-	4348591..4348667	-	77	NuclAT_11	-	Antitoxin
-	4348591..4348667	-	77	NuclAT_11	-	Antitoxin
-	4348591..4348667	-	77	NuclAT_11	-	Antitoxin
HHJ46_RS20945	4348662..4349003	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
HHJ46_RS20950	4349165..4350544	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
HHJ46_RS20955	4350544..4351590	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T154824 WP_000132601.1 NZ_CP051694:4348662-4349003 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T154824 NZ_CP068282:50094-50399 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT154824 NZ_CP051694:c4348667-4348591 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin