Detailed information of TA system TA154806

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1971173..1972004
• Replicon: chromosome
• Accession: NZ_CP051694
• Organism: Escherichia coli strain SCU-313

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A066T988
• Locus tag: HHJ46_RS09280
• Protein ID: WP_000854815.1
• Coordinates: 1971173..1971547 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A061Y7A8
• Locus tag: HHJ46_RS09285
• Protein ID: WP_001280918.1
• Coordinates: 1971636..1972004 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ46_RS09240	1966569..1967735	+	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
HHJ46_RS09245	1967854..1968327	+	474	WP_001105368.1	DNA gyrase inhibitor SbmC	-
HHJ46_RS09250	1968525..1969583	+	1059	WP_001200889.1	FUSC family protein	-
HHJ46_RS09255	1969755..1970084	+	330	WP_000450409.1	DUF496 family protein	-
HHJ46_RS09260	1970185..1970367	-	183	WP_001296208.1	hypothetical protein	-
HHJ46_RS09265	1970421..1970567	+	147	Protein_1813	transposase domain-containing protein	-
HHJ46_RS09270	1970856..1970969	-	114	WP_001161660.1	DUF957 domain-containing protein	-
HHJ46_RS09275	1970982..1971176	-	195	WP_000988600.1	hypothetical protein	-
HHJ46_RS09280	1971173..1971547	-	375	WP_000854815.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HHJ46_RS09285	1971636..1972004	-	369	WP_001280918.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ46_RS09290	1972020..1972664	-	645	WP_000086752.1	hypothetical protein	-
HHJ46_RS09295	1972683..1972904	-	222	WP_000692345.1	DUF987 domain-containing protein	-
HHJ46_RS09300	1972967..1973443	-	477	WP_001186200.1	RadC family protein	-
HHJ46_RS09305	1973459..1973932	-	474	WP_001542276.1	antirestriction protein	-
HHJ46_RS09310	1974026..1974271	-	246	WP_001164966.1	hypothetical protein	-
HHJ46_RS09315	1974271..1975089	-	819	WP_001542275.1	DUF945 domain-containing protein	-
HHJ46_RS09320	1975310..1975720	-	411	WP_000846703.1	hypothetical protein	-
HHJ46_RS09325	1975736..1976086	-	351	Protein_1825	hypothetical protein	-
HHJ46_RS09330	1976169..1976915	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1963875..2013190	49315

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13829.83 Da
• Isoelectric Point: 7.1326

>T154806 WP_000854815.1 NZ_CP051694:c1971547-1971173 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T154806 NZ_CP068281:c2513646-2513539 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13811.70 Da
• Isoelectric Point: 6.4767

>AT154806 WP_001280918.1 NZ_CP051694:c1972004-1971636 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT154806 NZ_CP068281:2513693-2513759 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A066T988

Antitoxin

• AlphaFold DB: A0A061Y7A8