Detailed information of TA system TA154803

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 835155..835989
• Replicon: chromosome
• Accession: NZ_CP051694
• Organism: Escherichia coli strain SCU-313

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: HHJ46_RS03995
• Protein ID: WP_000854820.1
• Coordinates: 835155..835532 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: HHJ46_RS04000
• Protein ID: WP_001285610.1
• Coordinates: 835621..835989 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ46_RS03965	830478..831626	-	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-
HHJ46_RS03970	831698..832681	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
HHJ46_RS03975	833492..833662	-	171	Protein_781	IS110 family transposase	-
HHJ46_RS03980	834091..834651	-	561	Protein_782	DUF4942 domain-containing protein	-
HHJ46_RS03985	834736..834933	-	198	WP_085949158.1	DUF957 domain-containing protein	-
HHJ46_RS03990	834961..835158	-	198	Protein_784	hypothetical protein	-
HHJ46_RS03995	835155..835532	-	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HHJ46_RS04000	835621..835989	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ46_RS04005	836069..836290	-	222	WP_000692347.1	DUF987 domain-containing protein	-
HHJ46_RS04010	836377..836853	-	477	WP_001186193.1	RadC family protein	-
HHJ46_RS04015	836868..837347	-	480	WP_000706978.1	antirestriction protein	-
HHJ46_RS04020	837613..838431	-	819	WP_001175155.1	DUF945 domain-containing protein	-
HHJ46_RS04025	838521..838754	-	234	WP_000883181.1	DUF905 family protein	-
HHJ46_RS04030	838760..839437	-	678	WP_001097565.1	hypothetical protein	-
HHJ46_RS04035	839588..840268	-	681	WP_001278649.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T154803 WP_000854820.1 NZ_CP051694:c835532-835155 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T154803 NZ_CP068281:c1676546-1676328 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT154803 WP_001285610.1 NZ_CP051694:c835989-835621 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT154803 NZ_CP068281:c1676946-1676572 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6