Detailed information of TA system TA154787

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 115624..116048
• Replicon: plasmid pSCU-387-1
• Accession: NZ_CP051689
• Organism: Escherichia coli strain SCU-387

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: HHJ48_RS26355
• Protein ID: WP_000775238.1
• Coordinates: 115624..115785 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	115831..116048 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ48_RS26320	110636..110863	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
HHJ48_RS26325	111000..111671	-	672	WP_115766405.1	conjugal transfer transcriptional regulator TraJ	-
HHJ48_RS26330	111865..112248	-	384	WP_001354030.1	relaxosome protein TraM	-
HHJ48_RS26335	112571..113173	+	603	WP_001323326.1	transglycosylase SLT domain-containing protein	-
HHJ48_RS26340	113469..114290	-	822	WP_162825039.1	DUF945 domain-containing protein	-
HHJ48_RS26345	114409..114696	-	288	WP_000107526.1	hypothetical protein	-
HHJ48_RS26355	115624..115785	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	115831..116048	-	218	NuclAT_0	-	Antitoxin
-	115831..116048	-	218	NuclAT_0	-	Antitoxin
-	115831..116048	-	218	NuclAT_0	-	Antitoxin
-	115831..116048	-	218	NuclAT_0	-	Antitoxin
HHJ48_RS26360	116017..116778	-	762	Protein_110	plasmid SOS inhibition protein A	-
HHJ48_RS26365	117285..119229	-	1945	Protein_111	ParB/RepB/Spo0J family partition protein	-
HHJ48_RS26370	119291..119523	-	233	Protein_112	DUF905 family protein	-
HHJ48_RS26375	119650..120103	-	454	Protein_113	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroB / iroC / iroD / iroE / iroN / senB	1..139635	139635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T154787 WP_000775238.1 NZ_CP051689:c115785-115624 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T154787 NZ_CP068279:c4052101-4051766 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 218 bp

>AT154787 NZ_CP051689:c116048-115831 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGGAAAGTGAGTGTGGTCAGTGCGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTCGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin