Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4070982..4071636 Replicon chromosome
Accession NZ_CP051688
Organism Escherichia coli strain SCU-387

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HHJ48_RS19700 Protein ID WP_000244781.1
Coordinates 4070982..4071389 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HHJ48_RS19705 Protein ID WP_000354046.1
Coordinates 4071370..4071636 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ48_RS19680 4066939..4068672 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
HHJ48_RS19685 4068678..4069388 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HHJ48_RS19690 4069413..4070309 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HHJ48_RS19695 4070421..4070942 + 522 WP_001055874.1 flavodoxin FldB -
HHJ48_RS19700 4070982..4071389 - 408 WP_000244781.1 protein YgfX Toxin
HHJ48_RS19705 4071370..4071636 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HHJ48_RS19710 4071879..4072859 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
HHJ48_RS19715 4072936..4073595 - 660 WP_000250274.1 hemolysin III family protein -
HHJ48_RS19720 4073759..4074070 - 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
HHJ48_RS19725 4074115..4075548 + 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T154778 WP_000244781.1 NZ_CP051688:c4071389-4070982 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T154778 NZ_CP068279:2657530-2657706 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT154778 WP_000354046.1 NZ_CP051688:c4071636-4071370 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT154778 NZ_CP068279:2657752-2658168 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References