Detailed information of TA system TA154765

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1567715..1568519
• Replicon: chromosome
• Accession: NZ_CP051688
• Organism: Escherichia coli strain SCU-387

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HHJ48_RS07440
• Protein ID: WP_115766378.1
• Coordinates: 1567715..1568095 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MN76
• Locus tag: HHJ48_RS07445
• Protein ID: WP_015953067.1
• Coordinates: 1568142..1568519 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ48_RS07410	1562984..1563643	-	660	WP_001159065.1	glutamine ABC transporter permease GlnP	-
HHJ48_RS07415	1563956..1564711	-	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-
HHJ48_RS07420	1564708..1566207	-	1500	WP_000174399.1	IS21-like element ISEc10 family transposase	-
HHJ48_RS07425	1566363..1567211	-	849	WP_001280489.1	DUF4942 domain-containing protein	-
HHJ48_RS07430	1567296..1567493	-	198	WP_023563519.1	DUF957 domain-containing protein	-
HHJ48_RS07440	1567715..1568095	-	381	WP_115766378.1	TA system toxin CbtA family protein	Toxin
HHJ48_RS07445	1568142..1568519	-	378	WP_015953067.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HHJ48_RS07450	1568598..1568819	-	222	WP_000692329.1	DUF987 domain-containing protein	-
HHJ48_RS07455	1568882..1569358	-	477	WP_001186726.1	RadC family protein	-
HHJ48_RS07460	1569373..1569858	-	486	WP_021522473.1	antirestriction protein	-
HHJ48_RS07465	1569949..1570767	-	819	WP_077532164.1	DUF945 domain-containing protein	-
HHJ48_RS07470	1570857..1571090	-	234	WP_001278280.1	DUF905 family protein	-
HHJ48_RS07475	1571096..1571773	-	678	WP_001097307.1	hypothetical protein	-
HHJ48_RS07480	1571921..1572601	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14072.88 Da
• Isoelectric Point: 5.5666

>T154765 WP_115766378.1 NZ_CP051688:c1568095-1567715 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T154765 NZ_CP068278:2262396-2262650 [Corynebacterium aquatimens]
ATGCATTTGGTATGGGATCTCAATGCCTGGGAGGACTACACCTACTGGCAAAACGAGGATCGGAAGACGCTAAAGCGGAT
CAATCAGCTACTGAAAGATATGCTGCGGGATCCGTTTGAGGGAATTGGCAAACCGGAGCCCTTGAAATACGGTGCCGAGG
GGGCTTGGTCGCGTCGAATCACCCAGGAACACCGTCTCGTCTATCTCGTCGAGGGCCGAGATCTCGTCATCCTGCAAGCC
CGTTACCACTACTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13766.60 Da
• Isoelectric Point: 6.2021

>AT154765 WP_015953067.1 NZ_CP051688:c1568519-1568142 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT154765 NZ_CP068278:2262145-2262396 [Corynebacterium aquatimens]
ATGACGATGAGCGCTAGTGAAGCTCGCCGAACGTTTTTTCCGCTGCTTGAGCAGGTTAATAGCGATCGGGAAGCTATCGA
GGTGGCTTCCCGCCACGGGAACGTAGTCATCATGCCGGCTGATGACTATGCCGCGTGGGTAGAAACCGCTTACCTCTTCA
GGTCACCAGCGAATGCGCGTCGACTGATGGAGTCTTACGAAAACGCTCGCGCCGGGCGGTTTGAGGAACACGAACTCGAC
CTTGATGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5Z3CIS0