Detailed information of TA system TA154753

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 402340..403174
• Replicon: chromosome
• Accession: NZ_CP051688
• Organism: Escherichia coli strain SCU-387

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: HHJ48_RS01905
• Protein ID: WP_000854689.1
• Coordinates: 402340..402717 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HHJ48_RS01910
• Protein ID: WP_001545732.1
• Coordinates: 402794..403174 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ48_RS01865	397590..398987	+	1398	WP_001540536.1	PTS ascorbate transporter subunit IIC	-
HHJ48_RS01870	399003..399308	+	306	WP_000218362.1	PTS ascorbate transporter subunit IIB	-
HHJ48_RS01875	399318..399782	+	465	WP_000776510.1	PTS ascorbate transporter subunit IIA	-
HHJ48_RS01880	399796..400446	+	651	WP_000056760.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-
HHJ48_RS01885	400456..400659	+	204	WP_001545728.1	hypothetical protein	-
HHJ48_RS01890	400718..401536	-	819	WP_001545729.1	DUF4942 domain-containing protein	-
HHJ48_RS01895	401650..401838	-	189	WP_096972724.1	DUF957 domain-containing protein	-
HHJ48_RS01900	401855..402343	-	489	WP_000761698.1	hypothetical protein	-
HHJ48_RS01905	402340..402717	-	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
HHJ48_RS01910	402794..403174	-	381	WP_001545732.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ48_RS01915	403224..403868	-	645	WP_001545733.1	hypothetical protein	-
HHJ48_RS01920	403887..404108	-	222	WP_000692330.1	DUF987 domain-containing protein	-
HHJ48_RS01925	404171..404647	-	477	WP_001186727.1	RadC family protein	-
HHJ48_RS01930	404663..405136	-	474	WP_001350782.1	antirestriction protein	-
HHJ48_RS01935	405478..406296	-	819	WP_169062247.1	DUF945 domain-containing protein	-
HHJ48_RS01940	406397..406606	-	210	Protein_371	DUF905 family protein	-
HHJ48_RS01945	406707..407162	-	456	WP_021528086.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T154753 WP_000854689.1 NZ_CP051688:c402717-402340 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T154753 NZ_CP068249:c332607-332512 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13994.73 Da
• Isoelectric Point: 5.0696

>AT154753 WP_001545732.1 NZ_CP051688:c403174-402794 [Escherichia coli]
VSDTLSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFNNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT154753 NZ_CP068249:332413-332477 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT