Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3614858..3615378 Replicon chromosome
Accession NZ_CP051615
Organism Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag HHM36_RS17180 Protein ID WP_001160964.1
Coordinates 3615094..3615378 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HHM36_RS17175 Protein ID WP_000125566.1
Coordinates 3614858..3615091 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM36_RS17150 3609931..3610218 + 288 WP_000203744.1 ferredoxin-like protein FixX -
HHM36_RS17155 3610277..3611608 + 1332 WP_001183209.1 MFS transporter -
HHM36_RS17160 3611716..3612246 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HHM36_RS17165 3612239..3614101 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
HHM36_RS17170 3614293..3614772 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HHM36_RS17175 3614858..3615091 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HHM36_RS17180 3615094..3615378 + 285 WP_001160964.1 CcdB family protein Toxin
HHM36_RS17185 3615405..3616253 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HHM36_RS17190 3616260..3616637 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HHM36_RS17195 3616640..3617461 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HHM36_RS17200 3617458..3618447 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HHM36_RS17205 3618447..3619733 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T154489 WP_001160964.1 NZ_CP051615:3615094-3615378 [Escherichia coli O25b:H4-ST131]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T154489 NZ_CP068096:c5085810-5085714 [Serratia plymuthica]
AACAAGCCCTGCACAAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT154489 WP_000125566.1 NZ_CP051615:3614858-3615091 [Escherichia coli O25b:H4-ST131]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT154489 NZ_CP068096:5085706-5085854 [Serratia plymuthica]
AACAGCATAAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAA
GTTGGCATTTTGTGCAGGGCTTGTTCAGCCATGCACTTTAAGAGTAGCTTACCGCGCTAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References