Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3421796..3422631 Replicon chromosome
Accession NZ_CP051615
Organism Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag HHM36_RS16255 Protein ID WP_000854759.1
Coordinates 3422254..3422631 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag HHM36_RS16250 Protein ID WP_001295723.1
Coordinates 3421796..3422164 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM36_RS16225 3418911..3419069 + 159 WP_001323397.1 DUF905 family protein -
HHM36_RS16230 3419224..3420042 + 819 WP_001234738.1 DUF945 domain-containing protein -
HHM36_RS16235 3420384..3420857 + 474 WP_001350782.1 antirestriction protein -
HHM36_RS16240 3420873..3421349 + 477 WP_001186775.1 RadC family protein -
HHM36_RS16245 3421412..3421633 + 222 WP_000692345.1 DUF987 domain-containing protein -
HHM36_RS16250 3421796..3422164 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HHM36_RS16255 3422254..3422631 + 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
HHM36_RS16260 3422628..3423116 + 489 WP_000761690.1 hypothetical protein -
HHM36_RS16265 3423133..3423309 + 177 WP_000839286.1 DUF957 domain-containing protein -
HHM36_RS16270 3423409..3423567 + 159 WP_001467148.1 hypothetical protein -
HHM36_RS16275 3423931..3424107 + 177 Protein_3177 helix-turn-helix domain-containing protein -
HHM36_RS16280 3424880..3426520 + 1641 WP_001332039.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3408101..3435587 27486


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T154484 WP_000854759.1 NZ_CP051615:3422254-3422631 [Escherichia coli O25b:H4-ST131]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T154484 NZ_CP068096:537439-537642 [Serratia plymuthica]
ATGACTAAAATTGACTATCTGATGCGTTTACGTAAATGCACCACGATTGACACCCTGGAACGCGTTATTGAAAAAAATAA
GTATGAGCTTTCGGATGACGAACTAGAGCTGTTCTACTCTGCAGCGGACCACCGTTTGGCCGAGCTCACCATGAATAAGC
TGTACGATAAAATCCCCGTTGCCGTTTGGAAATTCGTACGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT154484 WP_001295723.1 NZ_CP051615:3421796-3422164 [Escherichia coli O25b:H4-ST131]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT154484 NZ_CP068096:537020-537388 [Serratia plymuthica]
ATGGATGAGTATTCGCCTAAAAGGCACGATATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
AACGCTGGGCGACAGCCACCACGGCTGGGTGAACGATCCAACGTCTTCCGTCAACCTCCAGCTTAACGAGTTGATTGAAC
ATATTGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAAAGTGATTTATCGGAGTTAGTCGAAGAGTATCTC
GACGATACCTACACGCTGTTTAGCAGTTATGGTATTAATGATTCCGATTTGCGCCGCTGGCAAAAAACCAAAGCGCGATT
ATTCAGAATGTTCTCAGGAGAGGGCATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References