Detailed information of TA system TA154477

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1857801..1858635
• Replicon: chromosome
• Accession: NZ_CP051615
• Organism: Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: HHM36_RS08850
• Protein ID: WP_000854690.1
• Coordinates: 1858258..1858635 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: HHM36_RS08845
• Protein ID: WP_001305076.1
• Coordinates: 1857801..1858169 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHM36_RS08805	1852883..1854010	+	1128	Protein_1728	hypothetical protein	-
HHM36_RS08810	1854086..1854541	+	456	WP_000581502.1	hypothetical protein	-
HHM36_RS08815	1854620..1854853	+	234	WP_000902034.1	DUF905 family protein	-
HHM36_RS08820	1854954..1855772	+	819	WP_001234620.1	DUF945 domain-containing protein	-
HHM36_RS08825	1855827..1856312	+	486	WP_000849565.1	antirestriction protein	-
HHM36_RS08830	1856328..1856804	+	477	WP_001186726.1	RadC family protein	-
HHM36_RS08835	1856867..1857088	+	222	WP_000692329.1	DUF987 domain-containing protein	-
HHM36_RS08840	1857107..1857751	+	645	WP_000094916.1	hypothetical protein	-
HHM36_RS08845	1857801..1858169	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHM36_RS08850	1858258..1858635	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
HHM36_RS08855	1858632..1859120	+	489	WP_000761699.1	hypothetical protein	-
HHM36_RS08860	1859137..1859334	+	198	WP_000839293.1	DUF957 domain-containing protein	-
HHM36_RS08865	1859419..1860264	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
HHM36_RS08870	1860333..1860728	+	396	WP_000208384.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
HHM36_RS08875	1860721..1861654	+	934	Protein_1742	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HHM36_RS08880	1862086..1862241	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / ugd / kpsT	1813523..1879844	66321
	-	flank	IS/Tn	-	-	1862086..1862241	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T154477 WP_000854690.1 NZ_CP051615:1858258-1858635 [Escherichia coli O25b:H4-ST131]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T154477 NZ_CP068090:4237828-4237931 [Shigella boydii]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT154477 WP_001305076.1 NZ_CP051615:1857801-1858169 [Escherichia coli O25b:H4-ST131]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT154477 NZ_CP068090:c4237932-4237699 [Shigella boydii]
TAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCA
TTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAAT
AAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAACCGCCCGTTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8