Detailed information of TA system TA154063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 5026638..5027228
• Replicon: chromosome
• Accession: NZ_CP051427
• Organism: Klebsiella michiganensis strain X2-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: J5V7J4
• Locus tag: HGI13_RS23730
• Protein ID: WP_009653321.1
• Coordinates: 5026896..5027228 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: HGI13_RS23725
• Protein ID: WP_109213543.1
• Coordinates: 5026638..5026895 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGI13_RS23705	5022536..5023141	-	606	WP_004852315.1	glutathione S-transferase family protein	-
HGI13_RS23710	5023321..5024247	+	927	WP_049078228.1	LysR substrate-binding domain-containing protein	-
HGI13_RS23715	5024285..5025283	-	999	WP_049078227.1	aldo/keto reductase	-
HGI13_RS23720	5025384..5026298	+	915	WP_086531142.1	LysR family transcriptional regulator	-
HGI13_RS23725	5026638..5026895	+	258	WP_109213543.1	antitoxin	Antitoxin
HGI13_RS23730	5026896..5027228	+	333	WP_009653321.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HGI13_RS23740	5027551..5029008	+	1458	WP_032743138.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HGI13_RS23750	5029971..5031425	-	1455	WP_004852297.1	AMP nucleosidase	-
HGI13_RS23755	5031557..5031814	-	258	WP_004852295.1	histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5016348..5027228	10880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11823.69 Da
• Isoelectric Point: 10.5834

>T154063 WP_009653321.1 NZ_CP051427:5026896-5027228 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGIKTTGVIRCDQPRTI
DIAARNGKRLERIPDAVVNDVLARLDAIFS

Nucleotide

• Length: 333 bp

>T154063 NZ_CP067307:c3942214-3941807 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9448.92 Da
• Isoelectric Point: 4.3219

>AT154063 WP_109213543.1 NZ_CP051427:5026638-5026895 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPYYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT154063 NZ_CP067307:c3942461-3942195 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: J5V7J4