Detailed information of TA system TA153909

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 117829..118255
• Replicon: plasmid pSCZE2
• Accession: NZ_CP051224
• Organism: Escherichia coli strain SCZE5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HF562_RS24095
• Protein ID: WP_001312861.1
• Coordinates: 117829..117987 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	118031..118255 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF562_RS24065	113203..113892	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
HF562_RS24070	114079..114462	-	384	WP_001151524.1	relaxosome protein TraM	-
HF562_RS24075	114783..115385	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HF562_RS24080	115682..116503	-	822	WP_001234426.1	DUF945 domain-containing protein	-
HF562_RS24085	116621..116908	-	288	WP_000107535.1	hypothetical protein	-
HF562_RS24095	117829..117987	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	118031..118255	-	225	NuclAT_0	-	Antitoxin
-	118031..118255	-	225	NuclAT_0	-	Antitoxin
-	118031..118255	-	225	NuclAT_0	-	Antitoxin
-	118031..118255	-	225	NuclAT_0	-	Antitoxin
HF562_RS24100	118067..118255	+	189	WP_001299721.1	hypothetical protein	-
HF562_RS24105	118267..118986	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
HF562_RS24110	118983..119417	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
HF562_RS24115	119472..119669	-	198	Protein_136	hypothetical protein	-
HF562_RS24120	119697..119930	-	234	WP_000005990.1	DUF905 family protein	-
HF562_RS24125	119998..120537	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
HF562_RS24970	120563..120769	-	207	WP_000275856.1	hypothetical protein	-
HF562_RS24135	121908..122879	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qacE / aadA2 / dfrA12 / oqxB / oqxA / mph(A) / aac(3)-IId / blaTEM-1A / floR / aph(3')-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..143259	143259

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T153909 WP_001312861.1 NZ_CP051224:c117987-117829 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T153909 NZ_CP067245:647227-648549 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT153909 NZ_CP051224:c118255-118031 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin