Detailed information of TA system TA153800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 42587..43136
• Replicon: plasmid pAfl69
• Accession: NZ_CP051207
• Organism: Dolichospermum flos-aquae CCAP 1403/13F

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HGD76_RS24610
• Protein ID: WP_168697587.1
• Coordinates: 42798..43136 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HGD76_RS24605
• Protein ID: WP_096666814.1
• Coordinates: 42587..42811 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGD76_RS24590	40864..41052	-	189	WP_168642901.1	hypothetical protein	-
HGD76_RS24595	41147..41767	-	621	WP_168697585.1	TenA family protein	-
HGD76_RS24600	41915..42469	+	555	WP_168697586.1	hypothetical protein	-
HGD76_RS24605	42587..42811	+	225	WP_096666814.1	CopG family transcriptional regulator	Antitoxin
HGD76_RS24610	42798..43136	+	339	WP_168697587.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HGD76_RS24615	43313..43462	-	150	Protein_34	UPF0175 family protein	-
HGD76_RS24620	43550..44092	-	543	WP_168697588.1	NAD(P)H-dependent oxidoreductase	-
HGD76_RS24625	44323..44973	+	651	WP_168697589.1	site-specific integrase	-
HGD76_RS24630	45298..46536	+	1239	WP_168697590.1	transposase	-
HGD76_RS24635	46694..47668	-	975	WP_168697591.1	fatty acid desaturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..68932	68932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12341.26 Da
• Isoelectric Point: 6.9782

>T153800 WP_168697587.1 NZ_CP051207:42798-43136 [Dolichospermum flos-aquae CCAP 1403/13F]
MKRGEIYFANLSPAVGSEMDKRRPVLIVSNDANNNASTTVTILPITSNMIRVYPFEVLLNPEDSGLSKTSKVQAQQIRTI
SKQRVLGDMVGCLSQELIDLVNDAIKLHLGLE

Nucleotide

• Length: 339 bp

>T153800 NZ_CP067101:1878089-1878349 [Bradyrhizobium diazoefficiens]
ATGAAAATCGTCTGGGATGAGCCGAAGCGACTTGCCAATCTCGACAAGCATGGGTTGGATTTCGCCGACCTCAACGAGAC
ATTTTTCGACAACGCGCTGGTCGTTCCCTCGCATAACAAGAGCAAGCGCTGGGTCGCGGTCGGCGTAAGCATCAGCGGCG
TCATCGTGGTCGTATTCGCCCGGCTGGGACGCGAAGGCGTCAGCATTATCAGCGTGCGACCGGCAAGCCGTAGCGAAAGG
AAACTCTATGCCGAACGCTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8495.58 Da
• Isoelectric Point: 4.1514

>AT153800 WP_096666814.1 NZ_CP051207:42587-42811 [Dolichospermum flos-aquae CCAP 1403/13F]
MQAEKLSISLPSSLVQFIENYKLTKGCKSRSQVIELAIELLRYQELEQAYREAAAEFNPEWDLTVGDGLTDETW

Nucleotide

• Length: 225 bp

>AT153800 NZ_CP067101:1878336-1878629 [Bradyrhizobium diazoefficiens]
ATGCCGAACGCTAGGAAGAGATCAGGCTACACCAAAGAGGATCTGCGCGCGGTCAGCGATAACCCGGAGCTGACAAAGGC
GGATTTCGCCAAGGCCAAGCCGTTCTCTGAAGTGTTCCCTGACCTCAGCGCGTCCATCCGGAAGGGCCGCGGCCCGAACA
AGTCGCCGACAAAAAAGCTCGTCTCCTTGCGCCTCAGCCCGGAGGTGGTCGAGCACTTCAAGTCGACCGGGCCGGGCTGG
CAATCGCGGATCGACGAAACCTTGCGCAAGGCAGTCAAGCGCAAGGCGTCGTGA