Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 192185..192744 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP050866 | ||
| Organism | Escherichia coli strain 8-3-Ti3 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | D2AJT2 |
| Locus tag | HFC66_RS26055 | Protein ID | WP_000421262.1 |
| Coordinates | 192469..192744 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A0M1UJH5 |
| Locus tag | HFC66_RS26050 | Protein ID | WP_038341659.1 |
| Coordinates | 192185..192469 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HFC66_RS26010 | 188174..188443 | - | 270 | Protein_220 | transposase | - |
| HFC66_RS26015 | 188558..189196 | - | 639 | WP_000607008.1 | type III secretion system effector OspI | - |
| HFC66_RS26020 | 189349..189441 | - | 93 | Protein_222 | IS1 family transposase | - |
| HFC66_RS26025 | 189494..190191 | + | 698 | Protein_223 | IS1 family transposase | - |
| HFC66_RS26030 | 190204..190602 | - | 399 | Protein_224 | IS1 family transposase | - |
| HFC66_RS26035 | 190938..191123 | + | 186 | Protein_225 | ATP-binding protein | - |
| HFC66_RS26040 | 191223..191432 | - | 210 | Protein_226 | peptidoglycan-binding protein | - |
| HFC66_RS26045 | 191482..191661 | - | 180 | Protein_227 | MFS transporter | - |
| HFC66_RS26050 | 192185..192469 | + | 285 | WP_038341659.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| HFC66_RS26055 | 192469..192744 | + | 276 | WP_000421262.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| HFC66_RS26450 | 192876..193013 | - | 138 | WP_000766699.1 | hypothetical protein | - |
| HFC66_RS26065 | 193120..193471 | - | 352 | Protein_231 | plasmid partitioning/stability family protein | - |
| HFC66_RS26070 | 193474..194042 | - | 569 | Protein_232 | plasmid segregation protein ParM | - |
| HFC66_RS26075 | 194050..195141 | + | 1092 | Protein_233 | IS66 family transposase | - |
| HFC66_RS26080 | 195336..196544 | - | 1209 | Protein_234 | IS91 family transposase | - |
| HFC66_RS26085 | 196525..196797 | - | 273 | WP_000019158.1 | hypothetical protein | - |
| HFC66_RS26090 | 196957..197175 | + | 219 | WP_000813626.1 | type II toxin-antitoxin system antitoxin CcdA | - |
| HFC66_RS26095 | 197177..197482 | + | 306 | WP_001159860.1 | type II toxin-antitoxin system toxin CcdB | - |
| HFC66_RS26100 | 197518..197613 | + | 96 | Protein_238 | transposase domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ipaH1.4 / ospE2 / senB / faeF / faeH / faeI / ospC1 / ospD3/senA / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG | 1..233800 | 233800 | |
| - | inside | IScluster/Tn | - | ospI / ipaH9.8 / ospG | 180698..201244 | 20546 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10766.48 Da Isoelectric Point: 9.9324
>T153429 WP_000421262.1 NZ_CP050866:192469-192744 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T153429 NZ_CP066904:c5400359-5399976 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10444.88 Da Isoelectric Point: 5.5648
>AT153429 WP_038341659.1 NZ_CP050866:192185-192469 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEECNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEECNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR
Download Length: 285 bp
>AT153429 NZ_CP066904:c5400601-5400359 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A822PVM3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0M1UJH5 |