Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1831037..1831627 Replicon chromosome
Accession NZ_CP050829
Organism Klebsiella pneumoniae strain Bckp067

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag HC709_RS09030 Protein ID WP_032421545.1
Coordinates 1831295..1831627 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag HC709_RS09025 Protein ID WP_032421546.1
Coordinates 1831037..1831294 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC709_RS27590 1826863..1827105 - 243 WP_040219557.1 hypothetical protein -
HC709_RS09005 1827372..1829120 + 1749 WP_117053756.1 hypothetical protein -
HC709_RS09010 1829199..1829756 + 558 WP_087638062.1 hypothetical protein -
HC709_RS09015 1830039..1830499 + 461 Protein_1736 hypothetical protein -
HC709_RS09020 1830496..1830702 + 207 WP_020805021.1 helix-turn-helix domain-containing protein -
HC709_RS09025 1831037..1831294 + 258 WP_032421546.1 antitoxin Antitoxin
HC709_RS09030 1831295..1831627 + 333 WP_032421545.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HC709_RS09045 1831949..1833385 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
HC709_RS09055 1833751..1835205 - 1455 WP_004148975.1 AMP nucleosidase -
HC709_RS09060 1835335..1835580 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11917.79 Da        Isoelectric Point: 10.0968

>T153235 WP_032421545.1 NZ_CP050829:1831295-1831627 [Klebsiella pneumoniae]
MDRGEIWLVSLDPTSGHEQSGKRPVLIVSKASFNKLTQLPVIVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T153235 NZ_CP066809:158664-158939 [Shigella flexneri 3a]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACACCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9175.62 Da        Isoelectric Point: 4.7468

>AT153235 WP_032421546.1 NZ_CP050829:1831037-1831294 [Klebsiella pneumoniae]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLVIEPQKRPHYSLEELLAQCDPHAEISEGGREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT153235 NZ_CP066809:158936-159295 [Shigella flexneri 3a]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References