Detailed information of TA system TA153204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4486998..4487808
• Replicon: chromosome
• Accession: NZ_CP050822
• Organism: Klebsiella pneumoniae strain Bckp091

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HC645_RS22280
• Protein ID: WP_135714660.1
• Coordinates: 4486998..4487531 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: HC645_RS22285
• Protein ID: WP_002887278.1
• Coordinates: 4487542..4487808 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC645_RS22275	4485829..4486950	+	1122	WP_002887282.1	cupin domain-containing protein	-
HC645_RS22280	4486998..4487531	-	534	WP_135714660.1	GNAT family N-acetyltransferase	Toxin
HC645_RS22285	4487542..4487808	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
HC645_RS22290	4487911..4489344	-	1434	WP_135714659.1	Cu(+)/Ag(+) sensor histidine kinase	-
HC645_RS22295	4489334..4490017	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
HC645_RS22300	4490189..4491574	+	1386	WP_167803432.1	efflux transporter outer membrane subunit	-
HC645_RS22305	4491592..4491936	+	345	WP_002887266.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19809.66 Da
• Isoelectric Point: 5.6984

>T153204 WP_135714660.1 NZ_CP050822:c4487531-4486998 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGNTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T153204 NZ_CP066804:1625955-1626272 [Pseudoalteromonas sp. LC2018020214]
ATGGCTGAAATAATCTGGACTAATCCAGCTTTGGGTGATCTTAACGATATTGCTGAATATATCGCATTAAGCAATTTGCA
GCCAGCGAAAAAATTGGTTTCCAAAATATTTGAAAAAGTTGAACGTCTCGAACCCTTTCCTGAATCAGGTAAAAAACCAA
TTGAGTTAGCTAACCTAAATTATCTTGAACTGGTCGTGAATCCTTGTCGTATATTTTACAAAAAAGATAACAATAAAGTA
TATATTTTGCACGTAATGAGACAAGAACGAGACTTACGAAAATTTTTGCTGCTAACACAATATACAGCTAACAAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT153204 WP_002887278.1 NZ_CP050822:c4487808-4487542 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT153204 NZ_CP066804:1625710-1625967 [Pseudoalteromonas sp. LC2018020214]
ATGAGAGTAGAACTAGTAACAACATTGAAGCGTCAAGCAACGAAGATATTATCTGAACTTCATTCCTCTAAAGAACCTGT
GTTGATAACTGAACATGGACAACCATCTGCATACCTAGTAGATGTTGATGATTATGAATTTATGCAAAAGCGTATGCAAA
TTCTTGAGTCTCTTGCAAAAGGAGAGCAGGCAATAAGTCGTGGTGAAACCATGTCGAATGTTGAAGCTAAAGATAAAATG
AATAAATGGCTGAAATAA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1