Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4346971..4347596 Replicon chromosome
Accession NZ_CP050646
Organism Escherichia coli strain PapM-36-2

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag G6008_RS20780 Protein ID WP_000911329.1
Coordinates 4347198..4347596 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag G6008_RS20775 Protein ID WP_000450524.1
Coordinates 4346971..4347198 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6008_RS20750 4342773..4343243 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
G6008_RS20755 4343243..4343815 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
G6008_RS20760 4343961..4344839 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
G6008_RS20765 4344856..4345890 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
G6008_RS20770 4346103..4346816 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
G6008_RS20775 4346971..4347198 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
G6008_RS20780 4347198..4347596 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
G6008_RS20785 4347743..4348606 + 864 WP_001267498.1 neutral zinc metallopeptidase -
G6008_RS20790 4348621..4350636 + 2016 WP_167778832.1 tRNA cytosine(34) acetyltransferase TmcA -
G6008_RS20795 4350710..4351408 + 699 WP_000679812.1 esterase -
G6008_RS20800 4351489..4351689 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T153064 WP_000911329.1 NZ_CP050646:4347198-4347596 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T153064 NZ_CP066753:1053378-1053713 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT153064 WP_000450524.1 NZ_CP050646:4346971-4347198 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT153064 NZ_CP066753:1053130-1053378 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References