Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1914848..1915438 Replicon chromosome
Accession NZ_CP050508
Organism Raoultella terrigena strain JH01

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag HCK03_RS08980 Protein ID WP_167691427.1
Coordinates 1915106..1915438 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag HCK03_RS08975 Protein ID WP_115192355.1
Coordinates 1914848..1915105 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCK03_RS08945 1910850..1911407 - 558 WP_041146304.1 helix-turn-helix transcriptional regulator -
HCK03_RS08950 1911751..1912497 - 747 WP_041146303.1 NAD(P)-binding domain-containing protein -
HCK03_RS08955 1912623..1912967 + 345 WP_206110948.1 winged helix-turn-helix transcriptional regulator -
HCK03_RS08960 1913392..1914024 + 633 WP_167691426.1 thymidylate kinase -
HCK03_RS08965 1914090..1914296 + 207 WP_143992019.1 hypothetical protein -
HCK03_RS08970 1914293..1914499 + 207 WP_071844950.1 helix-turn-helix domain-containing protein -
HCK03_RS08975 1914848..1915105 + 258 WP_115192355.1 antitoxin Antitoxin
HCK03_RS08980 1915106..1915438 + 333 WP_167691427.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HCK03_RS08990 1915899..1916816 + 918 WP_041146297.1 nitrogen assimilation transcriptional regulator NAC -
HCK03_RS08995 1916923..1917873 + 951 WP_041146296.1 HTH-type transcriptional regulator Cbl -
HCK03_RS09000 1917988..1918929 + 942 WP_041147688.1 L,D-transpeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11863.77 Da        Isoelectric Point: 10.7239

>T152979 WP_167691427.1 NZ_CP050508:1915106-1915438 [Raoultella terrigena]
MDRGEIWLVSLDPIAGHEQSGRRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTIGVIRCDQPRTI
DMAARNGKRLERIPDAVINEVLARLDAILS

Download         Length: 333 bp

>T152979 NZ_CP066706:775175-775396 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9351.81 Da        Isoelectric Point: 4.4475

>AT152979 WP_115192355.1 NZ_CP050508:1914848-1915105 [Raoultella terrigena]
MYTTRLKKVGGSVMLAVPPAVLKTLALSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT152979 NZ_CP066706:774875-775162 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References