Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ypjF-yeeU/CbtA-CbeA
Location 2738544..2739319 Replicon chromosome
Accession NZ_CP050498
Organism Escherichia coli strain RM13322

Toxin (Protein)


Gene name ypjF Uniprot ID A0A7V7JG91
Locus tag HCR07_RS14290 Protein ID WP_001193488.1
Coordinates 2738544..2738915 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7V7JFU6
Locus tag HCR07_RS14295 Protein ID WP_001059301.1
Coordinates 2738954..2739319 (-) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCR07_RS14275 2734502..2735218 + 717 WP_001295734.1 N-acetylneuraminic acid outer membrane channel NanC -
HCR07_RS14280 2735238..2736344 + 1107 WP_001298927.1 N-acetylneuraminate epimerase -
HCR07_RS14285 2736409..2737389 + 981 WP_000991444.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
HCR07_RS14290 2738544..2738915 - 372 WP_001193488.1 TA system toxin CbtA family protein Toxin
HCR07_RS14295 2738954..2739319 - 366 WP_001059301.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HCR07_RS14300 2739345..2739566 - 222 WP_000691983.1 DUF987 domain-containing protein -
HCR07_RS14305 2739563..2740105 - 543 WP_015740440.1 DNA repair protein RadC -
HCR07_RS14310 2740118..2740561 - 444 WP_001096016.1 antirestriction protein -
HCR07_RS14315 2740592..2741413 - 822 WP_001234408.1 DUF945 domain-containing protein -
HCR07_RS14320 2741560..2742006 - 447 WP_032162830.1 hypothetical protein -
HCR07_RS14325 2742078..2742530 - 453 WP_000734321.1 hypothetical protein -
HCR07_RS14330 2742566..2743282 - 717 WP_000174910.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimE / fimB 2731370..2746060 14690


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(17-117)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13832.94 Da        Isoelectric Point: 7.2419

>T152944 WP_001193488.1 NZ_CP050498:c2738915-2738544 [Escherichia coli]
MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFSNETTILEHIDAGVSLCDAVNFLVEKYELVRIDCNDC
SVMEKSPFITSIDILRARKASGLMKRNSHKTVTRVTAGRLQEL

Download         Length: 372 bp

>T152944 NZ_CP066631:c42900-42550 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13388.25 Da        Isoelectric Point: 5.9530

>AT152944 WP_001059301.1 NZ_CP050498:c2739319-2738954 [Escherichia coli]
MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLSDRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHPHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Download         Length: 366 bp

>AT152944 NZ_CP066631:c43157-42894 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7JG91

Antitoxin

Source ID Structure
AlphaFold DB A0A7V7JFU6

References