Detailed information of TA system TA152388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 195104..196672
• Replicon: plasmid pAsmA
• Accession: NZ_CP050188
• Organism: Aeromonas salmonicida subsp. masoucida strain RZ6S-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: G9457_RS23740
• Protein ID: WP_128313846.1
• Coordinates: 195365..196672 (+)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: G9457_RS23735
• Protein ID: WP_219934974.1
• Coordinates: 195104..195355 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9457_RS23705 (G9457_23785)	190179..191009	-	831	WP_232048272.1	hypothetical protein	-
G9457_RS23710 (G9457_23790)	191021..191662	-	642	WP_219934972.1	hypothetical protein	-
G9457_RS23715 (G9457_23795)	192010..192540	+	531	WP_128313844.1	hypothetical protein	-
G9457_RS23720 (G9457_23800)	192543..193547	+	1005	WP_219934973.1	tyrosine-type recombinase/integrase	-
G9457_RS23725 (G9457_23805)	193769..193948	+	180	WP_128313842.1	hypothetical protein	-
G9457_RS23730 (G9457_23810)	194017..194466	+	450	WP_128313841.1	hypothetical protein	-
G9457_RS23735 (G9457_23815)	195104..195355	+	252	WP_219934974.1	helix-turn-helix domain-containing protein	Antitoxin
G9457_RS23740 (G9457_23820)	195365..196672	+	1308	WP_128313846.1	type II toxin-antitoxin system HipA family toxin	Toxin
G9457_RS23745 (G9457_23825)	196669..197163	+	495	WP_128313839.1	hypothetical protein	-
G9457_RS23750 (G9457_23830)	197486..197731	-	246	WP_128313837.1	hypothetical protein	-
G9457_RS23755 (G9457_23835)	197932..199518	+	1587	WP_128313836.1	DUF2235 domain-containing protein	-
G9457_RS23760 (G9457_23840)	199523..199807	+	285	WP_234915199.1	hypothetical protein	-
G9457_RS23765 (G9457_23845)	199963..200235	+	273	WP_219939305.1	hypothetical protein	-
G9457_RS23770 (G9457_23850)	200922..201137	+	216	WP_034168403.1	DUF2158 domain-containing protein	-
G9457_RS23775 (G9457_23855)	201167..201406	+	240	WP_219935054.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / formA / tet(A) / aac(6')-Ib-cr / blaOXA-1 / catB3 / catA2 / ARR-3 / qacE / sul1	-	1..252699	252699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 47845.08 Da
• Isoelectric Point: 6.2421

>T152388 WP_128313846.1 NZ_CP050188:195365-196672 [Aeromonas salmonicida subsp. masoucida]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIELLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDCWQRPLGSTPTSHIFKLPIGRIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMLEMATKTEAVVA
TVAAELPAGFPEHISNAIFDGLSSQAAKILKGVAK

Nucleotide

• Length: 1308 bp

>T152388 NZ_CP066143:c2649769-2649667 [Klebsiella pneumoniae U-0608239]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9411.83 Da
• Isoelectric Point: 9.9484

>AT152388 WP_219934974.1 NZ_CP050188:195104-195355 [Aeromonas salmonicida subsp. masoucida]
MRVTSPTLLAAAVRDQRKISKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Nucleotide

• Length: 252 bp

>AT152388 NZ_CP066143:2649660-2649805 [Klebsiella pneumoniae U-0608239]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT