Detailed information of TA system TA152308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4529135..4530393
• Replicon: chromosome
• Accession: NZ_CP050149
• Organism: Pseudomonas aeruginosa strain CHA

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HBA22_RS20985
• Protein ID: WP_003123189.1
• Coordinates: 4529135..4529890 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HBA22_RS20990
• Protein ID: WP_003086329.1
• Coordinates: 4530025..4530393 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBA22_RS20975 (HBA22_20975)	4524308..4526851	+	2544	WP_003123188.1	acyl-homoserine-lactone acylase QuiP	-
HBA22_RS20980 (HBA22_20980)	4527006..4528367	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
HBA22_RS20985 (HBA22_20985)	4529135..4529890	-	756	WP_003123189.1	RES family NAD+ phosphorylase	Toxin
HBA22_RS20990 (HBA22_20990)	4530025..4530393	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HBA22_RS20995 (HBA22_20995)	4530466..4531752	-	1287	WP_003123190.1	FAD-binding oxidoreductase	-
HBA22_RS21000 (HBA22_21000)	4531808..4533301	-	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
HBA22_RS21005 (HBA22_21005)	4533476..4533952	+	477	WP_003112531.1	hypothetical protein	-
HBA22_RS21010	4533955..4534230	-	276	WP_173014646.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28267.46 Da
• Isoelectric Point: 8.3428

>T152308 WP_003123189.1 NZ_CP050149:c4529890-4529135 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGMRLQAPPFSDHEAALTDIA
DYRAPQKLGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T152308 NZ_CP066108:c1117733-1117515 [Enterobacter hormaechei]
ATGTCTGAAAAACCATTAACGAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTCGAACGTGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGCCTTGCTGAAC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT152308 WP_003086329.1 NZ_CP050149:c4530393-4530025 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT152308 NZ_CP066108:c1118135-1117761 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAATCATGGCTGGGTAAACGATCCAACGTCTGCTATCAATTTACAGCTCAACGAGCTGATTGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCAAACCCGGTTAGTCTTTCCTGTTAG

Structures

Antitoxin

• AlphaFold DB: V6AJU7