Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2354774..2355363 Replicon chromosome
Accession NZ_CP050120
Organism Deinococcus radiodurans strain BND-54

Toxin (Protein)


Gene name mazF Uniprot ID Q9RX98
Locus tag HAV35_RS11855 Protein ID WP_010887062.1
Coordinates 2354774..2355127 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q9RX99
Locus tag HAV35_RS11860 Protein ID WP_010887061.1
Coordinates 2355121..2355363 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HAV35_RS11825 2349987..2350166 + 180 WP_028328008.1 hypothetical protein -
HAV35_RS11830 2350287..2351062 + 776 Protein_2310 methyltransferase domain-containing protein -
HAV35_RS11835 2351218..2351442 + 225 WP_162177572.1 hypothetical protein -
HAV35_RS11840 2351623..2354090 - 2468 Protein_2312 ATP-dependent helicase HrpB -
HAV35_RS11845 2354087..2354500 - 414 WP_010887064.1 type II toxin-antitoxin system VapC family toxin -
HAV35_RS11850 2354497..2354721 - 225 WP_162177573.1 hypothetical protein -
HAV35_RS11855 2354774..2355127 - 354 WP_010887062.1 endoribonuclease MazF Toxin
HAV35_RS11860 2355121..2355363 - 243 WP_010887061.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HAV35_RS11865 2355542..2357023 + 1482 Protein_2317 carboxylesterase/lipase family protein -
HAV35_RS11870 2357071..2357700 - 630 WP_027479568.1 HAD family hydrolase -
HAV35_RS11875 2357702..2357881 - 180 WP_034349506.1 hypothetical protein -
HAV35_RS11880 2357904..2358866 - 963 WP_034349507.1 NAD(P)/FAD-dependent oxidoreductase -
HAV35_RS11885 2358887..2359570 - 684 WP_010887056.1 SDR family oxidoreductase -
HAV35_RS11890 2359567..2359938 - 372 Protein_2322 inorganic pyrophosphatase -
HAV35_RS11895 2359931..2360350 - 420 WP_010887054.1 DinB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Antitoxin PemI-like

(1-80)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 12450.24 Da        Isoelectric Point: 7.3219

>T152274 WP_010887062.1 NZ_CP050120:c2355127-2354774 [Deinococcus radiodurans]
MVSDYVPDAGHLVWLNFTPQAGHEQGGRRPALVLSPAAYNGVTGLMQACPVTSRAKGYPFEVTLPAHLGVSGVVLADHCR
SLDWRSRRAEQLAEAPADVLAEVRGKLGSLLGMSEKA

Download         Length: 354 bp

>T152274 NZ_CP066089:1580195-1580413 [Citrobacter koseri]
ATGTCCGATAAACCATTAACGAAAACAGATTATTTGATGCGTTTACGACGCTGCCAGACAATAGACACGCTGGAACGTGT
AATAGAGAAAAATAAATATGAATTGTCTGACAATGAATTGGCTGTATTTTACTCAGCAGCAGATCACCGTCTTGCAGAAT
TGACGATGAATAAGCTCTACGACAAGATTCCCACCTCCGTGTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8911.12 Da        Isoelectric Point: 4.3178

>AT152274 WP_010887061.1 NZ_CP050120:c2355363-2355121 [Deinococcus radiodurans]
MTSQIQKWGNSLALRIPKALAQQVGLTQSSEVELLLQDGQIVIRPVPARQYDLAALLAEMTPENLHGETDWGALEGREEW

Download         Length: 243 bp

>AT152274 NZ_CP066089:1579793-1580167 [Citrobacter koseri]
ATGGATGAGTACTCACCCAAAAGACATGATATCGCACAGCTTAAATTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAGGAAAGCAACCACGGTTGGGTTAATGACCCTACTTCGGCAATCAATCTTCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGCACAGATAGATGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTACGGCATTAATGCGCAGGATCTGCAAAAATGGCGAAAATCAGGAAACCGCCT
GTTTCGCTGCTTCGTCAATGCCACCAGGGCGAATCCTGTTAGCTTGTCATGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7D2M
PDB 7D2P
PDB 7DHP
PDB 7D2Q
PDB 7D28
PDB 7D2N

Antitoxin

Source ID Structure
PDB 7D2P
PDB 7D2Q
PDB 7D2N
AlphaFold DB Q9RX99

References