Detailed information of TA system TA152272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/VapC(toxin)
• Location: 2354087..2354721
• Replicon: chromosome
• Accession: NZ_CP050120
• Organism: Deinococcus radiodurans strain BND-54

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q9RX96
• Locus tag: HAV35_RS11845
• Protein ID: WP_010887064.1
• Coordinates: 2354087..2354500 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HAV35_RS11850
• Protein ID: WP_162177573.1
• Coordinates: 2354497..2354721 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAV35_RS11820	2349364..2349990	+	627	WP_010887068.1	single-stranded DNA-binding protein DdrA	-
HAV35_RS11825	2349987..2350166	+	180	WP_028328008.1	hypothetical protein	-
HAV35_RS11830	2350287..2351062	+	776	Protein_2310	methyltransferase domain-containing protein	-
HAV35_RS11835	2351218..2351442	+	225	WP_162177572.1	hypothetical protein	-
HAV35_RS11840	2351623..2354090	-	2468	Protein_2312	ATP-dependent helicase HrpB	-
HAV35_RS11845	2354087..2354500	-	414	WP_010887064.1	type II toxin-antitoxin system VapC family toxin	Toxin
HAV35_RS11850	2354497..2354721	-	225	WP_162177573.1	hypothetical protein	Antitoxin
HAV35_RS11855	2354774..2355127	-	354	WP_010887062.1	endoribonuclease MazF	-
HAV35_RS11860	2355121..2355363	-	243	WP_010887061.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HAV35_RS11865	2355542..2357023	+	1482	Protein_2317	carboxylesterase/lipase family protein	-
HAV35_RS11870	2357071..2357700	-	630	WP_027479568.1	HAD family hydrolase	-
HAV35_RS11875	2357702..2357881	-	180	WP_034349506.1	hypothetical protein	-
HAV35_RS11880	2357904..2358866	-	963	WP_034349507.1	NAD(P)/FAD-dependent oxidoreductase	-
HAV35_RS11885	2358887..2359570	-	684	WP_010887056.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14634.68 Da
• Isoelectric Point: 5.5820

>T152272 WP_010887064.1 NZ_CP050120:c2354500-2354087 [Deinococcus radiodurans]
MTESTVLDANILSALLRSEANPLSIARQLSALAREGSLVVYAELLAGPEVTPDFLKGFLQEVDVQILPPSGLAVWESAGL
AYGSYAQRRQRSGGGSPRQILSDFVIGAHALYHGAALFTADPQHYRLSFPALTVLTP

Nucleotide

• Length: 414 bp

>T152272 NZ_CP066089:508355-508531 [Citrobacter koseri]
GTGAAGCAGAGCGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTCGATGTAACAAATGGAACGAATCATTTGAAGTTGAG
ATATCATGACAAACGAAGCGTCATGCCCAGACATCCTGGTGATGAAATTAAAGACACCCTGCGTAAAGCAATACTAAAGC
AGCTTGGTTTGAATTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8229.23 Da
• Isoelectric Point: 4.3481

>AT152272 WP_162177573.1 NZ_CP050120:c2354721-2354497 [Deinococcus radiodurans]
MTTLEARIENGKVVLTPEQAAELGLREGEVLPVTIAPHEAETEGNPFTRWIGTLPPLPDGEESARFYRRLRDGE

Nucleotide

• Length: 225 bp

>AT152272 NZ_CP066089:508578-508994 [Citrobacter koseri]
ATGCGTTATCCCGTCAATCTCATTCCCGCAGAAGAAGGGGGTTATGTTGTGTCGTTCCCGGACATTCCAGAGGCGTTAAC
ACAAGGTGACACCCGCCATGAGTCTCTCGAAGCCGCGCAGGGCGCATTAGTCACCGCCTTTGAATTTTATTTTGAAGATA
ACCAGCCAATTCCTTTACCTTCAATGGTTGATGAAGCTGATGATTATGTGGAGATTCCTCTAAGCATTGCGTCTAAAGTC
CTTTTGTTGAATGCGTTCCTTGAATCAAAAATCACGCAGCAGGAGCTGGCGAGGCGCATTGGCAGACCCAAGCAGGAAAT
TACCCGACTATTCGACTTGAAACACGCAACGAAAATTGATGCTGTACAGATTGCGGCCAAAGCATTGGGTAAAGAGTTAT
CGCTGACTATGCTTTAG

Structures

Toxin

• AlphaFold DB: Q9RX96