Detailed information of TA system TA152184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2395887..2396626
• Replicon: chromosome
• Accession: NZ_CP050067
• Organism: Klebsiella aerogenes strain 035

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HA513_RS11580
• Protein ID: WP_047048975.1
• Coordinates: 2396141..2396626 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: HA513_RS11575
• Protein ID: WP_015705366.1
• Coordinates: 2395887..2396153 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HA513_RS11555	2392179..2392796	+	618	WP_045360840.1	response regulator transcription factor	-
HA513_RS11560	2392892..2393389	+	498	WP_045360838.1	heme-binding protein	-
HA513_RS11565	2393794..2394324	+	531	WP_166479691.1	N-acetyltransferase	-
HA513_RS11570	2394379..2395614	-	1236	WP_020078885.1	MFS transporter	-
HA513_RS11575	2395887..2396153	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
HA513_RS11580	2396141..2396626	+	486	WP_047048975.1	GNAT family N-acetyltransferase	Toxin
HA513_RS11585	2396698..2398314	+	1617	WP_047048973.1	FAD-NAD(P)-binding protein	-
HA513_RS11590	2398433..2399533	+	1101	WP_015366663.1	NADH:flavin oxidoreductase/NADH oxidase	-
HA513_RS11595	2399590..2399748	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
HA513_RS11600	2400009..2401079	+	1071	WP_015705361.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17587.26 Da
• Isoelectric Point: 8.8793

>T152184 WP_047048975.1 NZ_CP050067:2396141-2396626 [Klebsiella aerogenes]
MGKISAPAPLSSHHQIAEFCCGETVLDQWLKQRGLKNQAQGAARTFVVCKEESHQVVGFYSLATGSVNHTEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T152184 NZ_CP066032:c740970-740797 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT152184 WP_015705366.1 NZ_CP050067:2395887-2396153 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT152184 NZ_CP066032:c741294-740998 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9