Detailed information of TA system TA152165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 4401915..4403610
• Replicon: chromosome
• Accession: NZ_CP050053
• Organism: Pseudomonas aeruginosa strain LIUYANG-C

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: HAP36_RS20360
• Protein ID: WP_003112901.1
• Coordinates: 4402741..4403610 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: HAP36_RS20355
• Protein ID: WP_003112902.1
• Coordinates: 4401915..4402748 (-)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP36_RS20335	4397146..4398630	-	1485	WP_003112906.1	aldehyde dehydrogenase	-
HAP36_RS20340	4398642..4399445	-	804	WP_003112905.1	aspartate dehydrogenase	-
HAP36_RS20345	4399456..4401138	-	1683	WP_003112904.1	thiamine pyrophosphate-binding protein	-
HAP36_RS20350	4401131..4401928	-	798	WP_003112903.1	SDR family oxidoreductase	-
HAP36_RS20355	4401915..4402748	-	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
HAP36_RS20360	4402741..4403610	-	870	WP_003112901.1	alpha/beta hydrolase	Toxin
HAP36_RS20365	4403619..4404149	-	531	WP_003112900.1	cupin domain-containing protein	-
HAP36_RS20370	4404163..4404924	-	762	WP_003112899.1	SDR family oxidoreductase	-
HAP36_RS20375	4404930..4405706	-	777	WP_003112898.1	ABC transporter permease	-
HAP36_RS20380	4405703..4406710	-	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
HAP36_RS20385	4406751..4407602	-	852	WP_003130104.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T152165 WP_003112901.1 NZ_CP050053:c4403610-4402741 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T152165 NZ_CP066018:c1281580-1281278 [Cupriavidus necator]
ATTTGCGCGGGGAAGGGAACGGCGCATGACCCGCTGTGGCGGGTCCGGGAGTTGATTGCGGCAGGGCGGGTCAGCTTGAC
GATGGCAGCGTTCCAGGGCGCCAGGAACATGTGTCTCACCCGCGGCGACGTCCTTGATGTAGTCGCCAGGATCCAGCCCG
CCGACTTCTACAAAAGCATGACGACCTACGTCGACCATACCGTCTGGCAGGATGTGTATCGCCCATGCACATCGTATGGA
GCGATATACCTGAAGCTGACGGTGACCGAGGAAGTCCTGATTGTGTCGTTCAAGGCGCGCTAG

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT152165 WP_003112902.1 NZ_CP050053:c4402748-4401915 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT152165 NZ_CP066018:c1281246-1280845 [Cupriavidus necator]
ATGCGATGCCCGGTATGTGGAGGCGCCGAACTCGAAAGGGGGCGGAAAGACTTTGTCTATACCTACCGTGGCGAGTCCAC
GGTAATCGAAGCGGTCCTTGCCGACTGGTGTGCTCAGTGTGGAGAAGGTGTGCTGGGCCAGGAAGAGGAAGAGCGGATCG
GACCGCTGGCGCTCGCCTTCAACAAGCAAGTCAACGCCTCGCTTGTCGATCCGACTTACATCATGCAAGTCCGCAAGAAA
CTCCACCTCGACCAGCGCGAAGCCGCAGAGATCTTCGGCGGCGGGGTCAATGCATTCTCGCGCTACGAGACTGGCCGGAC
CAAGCCGCCGCTTGCGCTGGTAAAACTGCTCAGGCTGCTGGACCGCCACCCGGAACTGCTTGAGGAGGTCCGGGCGGCCT
GA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1