Detailed information of TA system TA152160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 747018..747613
• Replicon: chromosome
• Accession: NZ_CP050053
• Organism: Pseudomonas aeruginosa strain LIUYANG-C

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HAP36_RS03520
• Protein ID: WP_003113526.1
• Coordinates: 747018..747296 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HAP36_RS03525
• Protein ID: WP_003113527.1
• Coordinates: 747308..747613 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP36_RS03505	742444..743682	+	1239	WP_003113524.1	dipeptidase	-
HAP36_RS03510	743744..744391	+	648	WP_003095021.1	carbonate dehydratase	-
HAP36_RS03515	744461..746689	-	2229	WP_003113525.1	TonB-dependent receptor	-
HAP36_RS03520	747018..747296	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HAP36_RS03525	747308..747613	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HAP36_RS03530	747977..749050	-	1074	WP_003113528.1	serine/threonine protein kinase	-
HAP36_RS03535	749047..750096	-	1050	WP_003113529.1	serine/threonine protein kinase	-
HAP36_RS03540	750099..751196	-	1098	WP_169917322.1	protein phosphatase 2C domain-containing protein	-
HAP36_RS03545	751726..751998	+	273	WP_003115921.1	hypothetical protein	-
HAP36_RS03550	752210..752500	+	291	WP_033992920.1	DUF5447 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T152160 WP_003113526.1 NZ_CP050053:747018-747296 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152160 NZ_CP066015:c2491960-2491703 [Kytococcus sedentarius]
GTGCGCCTGGTCTGGGACGAGAACGCCTGGGAGGACTACCTCTGGTGGCAGGCCCAGGACCGCAAGGTGCTCAAGCGGAT
CAACACCCTGCTCAAGGACGTCGCCCGGAACGGCAACGAGGGCACCGGCAAGCCCGAGGCCCTGAAGCACAGCCTCGCCG
GGTACTGGTCCCGCCGCATCACCGACGAGCACCGCCTCGTCCACAAGGTCACCGAGGACGAGATCCGCATCGCAGCCTGC
CGGTACCACTACGGGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT152160 WP_003113527.1 NZ_CP050053:747308-747613 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT152160 NZ_CP066015:c2492219-2491962 [Kytococcus sedentarius]
GTGAAGACGCTCAGCTACACCGAGTCACGCCGGCGATACGCCGAGGTGCTCGACCACGTGGTGGACGACCGCGAGGAGGT
CGTCATCACCCGCGCCGGGCACGAGCCCGTCGTCATCGTGTCCCTGGAGGAGTACGAGTCCCTCCGCGAGACCGCCTACC
TCATGCGGTCCCCGGCCAACGCCCGCCGCCTGCTCGACGCCATGGACCGCCTCGAGGCCGGCGGCGGCACCGAGCACGAG
CTGATCGAGACCGACTGA

Structures

Toxin

• AlphaFold DB: V6ALY3