Detailed information of TA system TA152158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5253506..5254101
• Replicon: chromosome
• Accession: NZ_CP050052
• Organism: Pseudomonas aeruginosa strain LIUYANG-E

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HAP34_RS24260
• Protein ID: WP_003113526.1
• Coordinates: 5253823..5254101 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HAP34_RS24255
• Protein ID: WP_003113527.1
• Coordinates: 5253506..5253811 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP34_RS24230	5248619..5248909	-	291	WP_033992920.1	DUF5447 family protein	-
HAP34_RS24235	5249121..5249393	-	273	WP_003115921.1	hypothetical protein	-
HAP34_RS24240	5249923..5251020	+	1098	WP_169917322.1	protein phosphatase 2C domain-containing protein	-
HAP34_RS24245	5251023..5252072	+	1050	WP_003113529.1	serine/threonine protein kinase	-
HAP34_RS24250	5252069..5253142	+	1074	WP_003113528.1	serine/threonine protein kinase	-
HAP34_RS24255	5253506..5253811	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HAP34_RS24260	5253823..5254101	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HAP34_RS24265	5254430..5256658	+	2229	WP_003113525.1	TonB-dependent receptor	-
HAP34_RS24270	5256728..5257375	-	648	WP_003095021.1	carbonate dehydratase	-
HAP34_RS24275	5257437..5258675	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T152158 WP_003113526.1 NZ_CP050052:c5254101-5253823 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T152158 NZ_CP066015:c2075720-2075454 [Kytococcus sedentarius]
TTGGCCTGGACCGTTGAGGTCTCGGCGACGGCGCGCAAGGCGCTGAAGAAGCTGGACCCACCGGTGGCCCGCCGCATCGT
CCGCTTCCTGCGGGAGCTCGAGGCGCTGGACGATCCGCGGGCCCGAGGCAAGGCCCTCACGGGGGTTCTGTCCGGGCTGT
GGCGCTACCGGGTCGGGGACCACCGGGTCATCTGTGACGTGGACGACGAGGTCCTCGTCGTCCACGTCATCGAGGTCGGG
CACCGCTCCTCGGTGTACGACGACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT152158 WP_003113527.1 NZ_CP050052:c5253811-5253506 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT152158 NZ_CP066015:c2075937-2075704 [Kytococcus sedentarius]
ATGACCGTCGCCACCTCCGTGAAGCTGTCCGACGAGACCGGCCGCAAGCTCGAGCAGCTGGCCGCCGCCACCGGGCGCTC
GAAGTCCTACTACCTCCGCGAGGCCGTGGAGACCCACATCGACCGCCTCCTCTACGAGTACGGCATCGTCCAGCGGGTCG
AGGATGTCCGCGCCGGCCGCGAGGCCACCTACGGTCTCGACGAGGTGGAGGCGGATCTTGGCCTGGACCGTTGA

Structures

Toxin

• AlphaFold DB: V6ALY3