Detailed information of TA system TA152156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4397618..4398876
• Replicon: chromosome
• Accession: NZ_CP050052
• Organism: Pseudomonas aeruginosa strain LIUYANG-E

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: HAP34_RS20230
• Protein ID: WP_003112528.1
• Coordinates: 4397618..4398373 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HAP34_RS20235
• Protein ID: WP_003086329.1
• Coordinates: 4398508..4398876 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAP34_RS20220	4392791..4395334	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
HAP34_RS20225	4395489..4396850	-	1362	WP_010895536.1	DNA recombination protein RmuC	-
HAP34_RS20230	4397618..4398373	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
HAP34_RS20235	4398508..4398876	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HAP34_RS20240	4398949..4400235	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
HAP34_RS20245	4400291..4401880	-	1590	WP_010895535.1	aldehyde dehydrogenase family protein	-
HAP34_RS20250	4401959..4402435	+	477	WP_003112531.1	hypothetical protein	-
HAP34_RS20255	4402438..4402713	-	276	WP_010793902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T152156 WP_003112528.1 NZ_CP050052:c4398373-4397618 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T152156 NZ_CP066013:610408-610515 [Staphylococcus equorum]
GTGGTTCCAATTGTAGAAGCACTACACTTAATGTTAGGTTTCGGTACTTTCATCGTTACCTTATTAGGTTTAGTGGTTGC
AATTGTTAAATTAAGCCATAAAAAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT152156 WP_003086329.1 NZ_CP050052:c4398876-4398508 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT152156 NZ_CP066013:c610667-610505 [Staphylococcus equorum]
ATTTGATAATATTTTTATGTTGTGGTAATTTATATATAGAAAAAGGGCAACACACCATAAGTGTATCGCCCTAATGAGCC
CGTTAAAAAGACGGTGGCTGGAATTTAATAATGATTAAATAATCATCTCATCCTCGCCAAAGTTAGAGATGGTTATTTTT
TAT

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7