Detailed information of TA system TA152029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 2822330..2822907
• Replicon: chromosome
• Accession: NZ_CP050029
• Organism: Listeria monocytogenes strain CH3K-

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: G8O94_RS14240
• Protein ID: WP_003728465.1
• Coordinates: 2822330..2822671 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: G8O94_RS14245
• Protein ID: WP_003728464.1
• Coordinates: 2822665..2822907 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G8O94_RS14225	2820878..2821132	+	255	WP_031641560.1	helix-turn-helix transcriptional regulator	-
G8O94_RS14230	2821136..2822107	+	972	WP_031641941.1	hypothetical protein	-
G8O94_RS14235	2822161..2822304	+	144	WP_157105793.1	hypothetical protein	-
G8O94_RS14240	2822330..2822671	-	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G8O94_RS14245	2822665..2822907	-	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G8O94_RS14250	2822968..2823204	-	237	WP_003769265.1	hypothetical protein	-
G8O94_RS14255	2823350..2823676	+	327	WP_031641563.1	hypothetical protein	-
G8O94_RS14260	2823676..2824338	+	663	WP_003731676.1	DUF2786 domain-containing protein	-
G8O94_RS14265	2824357..2824734	+	378	WP_003769263.1	DUF3850 domain-containing protein	-
G8O94_RS15670	2825133..2825381	+	249	WP_009911232.1	hypothetical protein	-
G8O94_RS14275	2825573..2826502	+	930	WP_031672448.1	AAA family ATPase	-
G8O94_RS14280	2826531..2827211	-	681	WP_002389568.1	IS6 family transposase	-
G8O94_RS14285	2827287..2827403	-	117	Protein_2831	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	oatA / inlK	2653148..2852106	198958
	-	flank	IS/Tn	-	-	2826531..2827211	680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12827.74 Da
• Isoelectric Point: 9.2830

>T152029 WP_003728465.1 NZ_CP050029:c2822671-2822330 [Listeria monocytogenes]
MVKQGDIIKINLNPKQGHEQQGYRPYICLSHHLVSDYANIAIFAPISNTPRTYPLYVPLTGTTTTGKVLLDQLVTIDYNA
RKHQYVETVSDPLLTKLLDTVKVIFQKNERSTN

Nucleotide

• Length: 342 bp

>T152029 NZ_CP065926:819901-820122 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9207.48 Da
• Isoelectric Point: 4.3867

>AT152029 WP_003728464.1 NZ_CP050029:c2822907-2822665 [Listeria monocytogenes]
MTTVSVKKWGNSKAVRLPNNLLNALDIKENDDLKVEVINNQMILTKTAIDLRIEDLFANYQGEPFQTELQTFEPIGDEKW

Nucleotide

• Length: 243 bp

>AT152029 NZ_CP065926:819601-819888 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5