Detailed information of TA system TA152028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 1702764..1703341
• Replicon: chromosome
• Accession: NZ_CP050028
• Organism: Listeria monocytogenes strain C3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: G8O82_RS08525
• Protein ID: WP_003728465.1
• Coordinates: 1702764..1703105 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: G8O82_RS08530
• Protein ID: WP_003728464.1
• Coordinates: 1703099..1703341 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G8O82_RS08510	1701312..1701566	+	255	WP_031641560.1	helix-turn-helix transcriptional regulator	-
G8O82_RS08515	1701570..1702541	+	972	WP_031641941.1	hypothetical protein	-
G8O82_RS08520	1702595..1702738	+	144	WP_157105793.1	hypothetical protein	-
G8O82_RS08525	1702764..1703105	-	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G8O82_RS08530	1703099..1703341	-	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G8O82_RS08535	1703402..1703638	-	237	WP_003769265.1	hypothetical protein	-
G8O82_RS08540	1703784..1704110	+	327	WP_031641563.1	hypothetical protein	-
G8O82_RS08545	1704110..1704772	+	663	WP_003731676.1	DUF2786 domain-containing protein	-
G8O82_RS08550	1704791..1705168	+	378	WP_003769263.1	DUF3850 domain-containing protein	-
G8O82_RS15645	1705567..1705815	+	249	WP_009911232.1	hypothetical protein	-
G8O82_RS08560	1706007..1706936	+	930	WP_031672448.1	AAA family ATPase	-
G8O82_RS08565	1706965..1707645	-	681	WP_002389568.1	IS6 family transposase	-
G8O82_RS08570	1707721..1707840	-	120	Protein_1670	DDE-type integrase/transposase/recombinase	-
G8O82_RS08575	1707908..1708192	+	285	WP_223196619.1	phage holin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1706965..1707645	680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12827.74 Da
• Isoelectric Point: 9.2830

>T152028 WP_003728465.1 NZ_CP050028:c1703105-1702764 [Listeria monocytogenes]
MVKQGDIIKINLNPKQGHEQQGYRPYICLSHHLVSDYANIAIFAPISNTPRTYPLYVPLTGTTTTGKVLLDQLVTIDYNA
RKHQYVETVSDPLLTKLLDTVKVIFQKNERSTN

Nucleotide

• Length: 342 bp

>T152028 NZ_CP065926:195613-195879 [Staphylococcus pseudintermedius]
ATGGGTAATTATTCTGTCTTAATTAAGAATTCAGCCAAAAATGACTTGAAAAAAATTAAAAAGTCACTTCTTAAAGAGCA
ATTTTCAAAAATTATTGAAGTTTTAAAGGTTAACCCTTATGAAGCTAGTCAATCTTTTGAGAAGCTACAACCGAAGCATT
CAGCTCGATATTCAAGGCGTCTGAATCATCAACATAGAGTTGTGTATACGGTTGATGATGAAAAAAGAGAAGTTTATATT
TTTTCTGCTTGGTCATATTATGAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9207.48 Da
• Isoelectric Point: 4.3867

>AT152028 WP_003728464.1 NZ_CP050028:c1703341-1703099 [Listeria monocytogenes]
MTTVSVKKWGNSKAVRLPNNLLNALDIKENDDLKVEVINNQMILTKTAIDLRIEDLFANYQGEPFQTELQTFEPIGDEKW

Nucleotide

• Length: 243 bp

>AT152028 NZ_CP065926:195356-195613 [Staphylococcus pseudintermedius]
ATGATTATTAAAACGCCTACAAATGCTAGAAAAGAGTTTTACGAGTTGTTAAAACAAGTTAATGATAATCATGAGCCTAT
TATAATAAGTGGTTCTAAAAGTGAAAACAATGCAGTTATTGTTAGTCAGGAAGACTGGGATGGTATTCAAGAAACGTTAT
TTCTCGAATACACTGGTACTATGGATAAAGTAAGAGCACGTGAAAAAGATGATAGTGGTTATACCAATATTGACGATATA
GATTGGGATAATTTGTAA

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5