Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1652245..1653044 Replicon chromosome
Accession NZ_CP049845
Organism Escherichia coli strain UPEC-245U/190328

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag G9W35_RS07960 Protein ID WP_000347266.1
Coordinates 1652245..1652709 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag G9W35_RS07965 Protein ID WP_001307405.1
Coordinates 1652709..1653044 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W35_RS07930 (1647246) 1647246..1647680 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
G9W35_RS07935 (1647698) 1647698..1648576 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
G9W35_RS07940 (1648566) 1648566..1649345 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
G9W35_RS07945 (1649356) 1649356..1649829 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
G9W35_RS07950 (1649852) 1649852..1651132 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
G9W35_RS07955 (1651381) 1651381..1652190 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
G9W35_RS07960 (1652245) 1652245..1652709 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
G9W35_RS07965 (1652709) 1652709..1653044 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
G9W35_RS07970 (1653193) 1653193..1654764 - 1572 WP_001273741.1 galactarate dehydratase -
G9W35_RS07975 (1655139) 1655139..1656473 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
G9W35_RS07980 (1656489) 1656489..1657259 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T151679 WP_000347266.1 NZ_CP049845:c1652709-1652245 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T151679 NZ_CP065662:c122479-122126 [Aerococcus urinae]
ATGGTAATGCGGCAGGGCGACATTTTTTACGTTAATTTTAATCCAAATATCGGGCACGAACAGAGAAATAGTCGTCCGGC
TATTGTATTAAGTCATGATCTCATTTTTCAGACGAGTCGAATGGCGATTGTTGCGCCTATCTCAACAACTAAACGAAATT
ATCCAGCCTATTATGAGCTTCAAGAGACTGACCATACCAAGGGTAAAGTTCTTTTGGATCAAACAAAGTCTCTTGATCTT
TATCATTGCCAAGTCAGCCAAGAAAATTTTATTGAACGAGCTAAACCGAATGAATTTAAGCGTATTATTCATCGTTATAA
ATTGTTATTCGATCTGGTAGATGATTATCAATAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT151679 WP_001307405.1 NZ_CP049845:c1653044-1652709 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT151679 NZ_CP065662:c122715-122473 [Aerococcus urinae]
ATGCAAACTAAAATTGCTCAATGGGGGAATTCAAAGGCGATCAGAATCCCATCAACGATAGTTAAACAGCTCAATTTAAA
GGATAATCAAGTGCTCTCACTTGAAATCAAAGATCAGGCCCTAGTGATTAAGCCTAAGGAAGCAGAAACTATTCAGGAAT
TATTTGCTGATTACAACACTTCTTTCACTTATGAGGAAGAAATGGATTGGGGACCAGCAGTGGGAGAAGAGATCGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References