Detailed information of TA system TA151634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 20681..21237
• Replicon: plasmid pC
• Accession: NZ_CP049833
• Organism: Aeromonas salmonicida subsp. masoucida strain RZ6S-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2H9U809
• Locus tag: G9H43_RS24480
• Protein ID: WP_042468953.1
• Coordinates: 20681..20956 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: G9H43_RS24485
• Protein ID: WP_174416604.1
• Coordinates: 20956..21237 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9H43_RS24460 (G9H43_24595)	16601..17539	-	939	WP_258236885.1	IS630 family transposase	-
G9H43_RS24465 (G9H43_24600)	17827..18744	-	918	WP_076611341.1	IS5-like element ISAs4 family transposase	-
G9H43_RS24470 (G9H43_24605)	18793..18978	-	186	WP_088814096.1	hypothetical protein	-
G9H43_RS24475 (G9H43_24610)	18980..19894	-	915	WP_042468951.1	TOMM precursor leader peptide-binding protein	-
G9H43_RS24480 (G9H43_24615)	20681..20956	-	276	WP_042468953.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G9H43_RS24485 (G9H43_24620)	20956..21237	-	282	WP_174416604.1	ribbon-helix-helix domain-containing protein	Antitoxin
G9H43_RS24490 (G9H43_24625)	21449..22009	+	561	WP_042468955.1	recombinase family protein	-
G9H43_RS24495 (G9H43_24630)	22013..25041	+	3029	Protein_25	Tn3 family transposase	-
G9H43_RS24500 (G9H43_24635)	25076..25750	+	675	Protein_26	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	aopO / sycO / aopO / aopO / aopO / sycO / ascU / ascT / ascS / ascR / ascQ / ascP / ascO / ascN / aopN / acr1 / acr2 / ascX / ascY / ascV / acrR / acrG / acrV / acrH / aopB / aopD / exsC / exsE / exsB / exsA / exsA / exsD / ascB / ascC / ascD / ascE / ascF / ascG / ascH / ascI / ascJ / ascK / ascL / ati1 / ati2	1..67866	67866
	-	inside	IScluster/Tn	-	aopO / sycO / aopO / aopO / aopO / sycO	4704..34203	29499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-50)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10685.43 Da
• Isoelectric Point: 9.5291

>T151634 WP_042468953.1 NZ_CP049833:c20956-20681 [Aeromonas salmonicida subsp. masoucida]
MELKWTSKALSDLARLYEFLALANKPAAARAVQALTQAPTILLTNPRIGEQLFQFEPREVRRILVGEYEVRYEILDSIIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T151634 NZ_CP065624:c3296600-3296322 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10351.80 Da
• Isoelectric Point: 5.6624

>AT151634 WP_174416604.1 NZ_CP049833:c21237-20956 [Aeromonas salmonicida subsp. masoucida]
MKGNMTQATTKVLTAHVPLPMADKVDQMAARLERSRGWIMKQALSAWLAQEEDRDRLTQEALADVDAGRVIDHLAVQAWA
DSLNTDQPLSVPR

Nucleotide

• Length: 282 bp

>AT151634 NZ_CP065624:c3296863-3296603 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A2H9U809