Detailed information of TA system TA151481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 6145492..6147119
• Replicon: chromosome
• Accession: NZ_CP049737
• Organism: Pseudomonas sp. AN-B15

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GTQ41_RS27895
• Protein ID: WP_047297334.1
• Coordinates: 6145767..6147119 (-)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GTQ41_RS27890
• Protein ID: WP_047297333.1
• Coordinates: 6145492..6145770 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTQ41_RS27860 (GTQ41_27895)	6140942..6141565	+	624	WP_047297327.1	FMN-binding negative transcriptional regulator	-
GTQ41_RS27865 (GTQ41_27900)	6141576..6142022	+	447	WP_047297328.1	GNAT family N-acetyltransferase	-
GTQ41_RS27870 (GTQ41_27905)	6142035..6142706	+	672	WP_047297329.1	GNAT family protein	-
GTQ41_RS27875 (GTQ41_27910)	6142795..6143694	-	900	WP_047297330.1	homocysteine S-methyltransferase family protein	-
GTQ41_RS27880 (GTQ41_27915)	6143806..6144627	+	822	WP_047297331.1	ABC transporter substrate-binding protein	-
GTQ41_RS27885 (GTQ41_27920)	6144624..6145460	+	837	WP_047297332.1	amino acid ABC transporter permease	-
GTQ41_RS27890 (GTQ41_27925)	6145492..6145770	-	279	WP_047297333.1	helix-turn-helix transcriptional regulator	Antitoxin
GTQ41_RS27895 (GTQ41_27930)	6145767..6147119	-	1353	WP_047297334.1	HipA domain-containing protein	Toxin
GTQ41_RS27900 (GTQ41_27935)	6147621..6149879	+	2259	WP_217325397.1	autotransporter outer membrane beta-barrel domain-containing protein	-
GTQ41_RS27905 (GTQ41_27940)	6150052..6151860	-	1809	WP_007962245.1	sodium-extruding oxaloacetate decarboxylase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-368)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 50164.90 Da
• Isoelectric Point: 5.2697

>T151481 WP_047297334.1 NZ_CP049737:c6147119-6145767 [Pseudomonas sp. AN-B15]
MYNLTLQVFSAGKWHDAMILRFDNPEMGFESRCSFGYETPYLVENIEAIGSSFSKAVSALYPLDWEGRRSSTPAFVYDIA
PAGAAKRFLLARLGQEKPAHIGADLYLLGQSTPAPIGNMRVKESAEAVDEREPIGFKREDVVHRDSRFLEYAYEQGAAVG
GATGAGGEAPKLLLAENKAGLLYPDAVLGDEEVRQHWFIKFARNKGSLVDQDILRSEFHYYKALQKLGIETIAVEGLALE
EASKPSLWMQRFDREVTNGEVNRFAVESIYSLAEVTAPGSAMNHMDVIRMLAGLWRKAGQASQIPALVTDYLRRDLINKI
LGNSDNHGRNTAIIRQESSFRLAPIYDLAPMVMDDEGVTRTTKWPKDLERAGEVDWRGVCRALADITDPKDPLAGLTDAS
DPFERLREDAQRLAALPDILTASGLPDRTMNHPGIHLKNLEQRLKEWDLK

Nucleotide

• Length: 1353 bp

>T151481 NZ_CP065535:1428143-1428397 [Enterococcus durans]
TTGGGATACAATATAAAAATTAAGAAGAATGCCCGGAAAGACTTGCCCAAACTGAAAGGTGCCAATTTGAATGACAAGTT
TGATGAGCTAATTACAGTCATTAGAGAAAACCCTTATCAGAATCCACCACCTTATGAAAAGTTAATGTGGTTACTTTATT
ATTCGAGACGCATAAATATTAAATATCGTTTAGTTTATAGCGTGGAGGATCAAAATCTGTATGGCCTCATTATGAACACG
GGTTTTAAGATGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10370.13 Da
• Isoelectric Point: 10.9383

>AT151481 WP_047297333.1 NZ_CP049737:c6145770-6145492 [Pseudomonas sp. AN-B15]
MSMTVAERTVLIENIQEALAQGTLEVGEAVRRLRVEVTGLHQTQFARMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGL
KMGVVRIRREVN

Nucleotide

• Length: 279 bp

>AT151481 NZ_CP065535:1427907-1428158 [Enterococcus durans]
ATGGAAGTTATTTCTCCAACAAATGCGAGAAAAAATCTTTATGAAATCATCAAGCATGTTGTGTCAGACAGCCAGCCTAT
TGAAATTTCTAGTACAAAAGATGGCGAAAGTGTTGTTATGATTAGCAAAAGTGACTGGAACTCCTTACAAGAAACAATTT
ACTTACAGAATGCGGGTGTTTTAGATCAAATCCATCACTTTGAAAATGAAGAAAGTGAAGATTTGGGTGAAATTGATTGG
GATACAATATAA