Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1527762..1528385 Replicon chromosome
Accession NZ_CP049737
Organism Pseudomonas sp. AN-B15

Toxin (Protein)


Gene name hicA Uniprot ID W6VV48
Locus tag GTQ41_RS07015 Protein ID WP_007963251.1
Coordinates 1528206..1528385 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2A2PH83
Locus tag GTQ41_RS07010 Protein ID WP_047292246.1
Coordinates 1527762..1528169 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTQ41_RS06980 (GTQ41_06985) 1523089..1524438 + 1350 WP_047292230.1 GGDEF domain-containing protein -
GTQ41_RS06985 (GTQ41_06990) 1524557..1524715 + 159 WP_080963245.1 KGG domain-containing protein -
GTQ41_RS06990 (GTQ41_06995) 1524791..1525294 + 504 WP_047292232.1 DUF4142 domain-containing protein -
GTQ41_RS06995 (GTQ41_07000) 1525394..1525804 - 411 WP_027614440.1 low affinity iron permease family protein -
GTQ41_RS07000 (GTQ41_07005) 1525992..1526762 + 771 WP_047292235.1 trans-aconitate 2-methyltransferase -
GTQ41_RS07005 (GTQ41_07010) 1526886..1527614 - 729 WP_047292245.1 class I SAM-dependent methyltransferase -
GTQ41_RS07010 (GTQ41_07015) 1527762..1528169 - 408 WP_047292246.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GTQ41_RS07015 (GTQ41_07020) 1528206..1528385 - 180 WP_007963251.1 type II toxin-antitoxin system HicA family toxin Toxin
GTQ41_RS07025 (GTQ41_07030) 1528885..1529769 - 885 WP_047292252.1 alpha/beta hydrolase -
GTQ41_RS07030 (GTQ41_07035) 1530086..1530778 - 693 WP_047292253.1 pseudouridine synthase -
GTQ41_RS07035 (GTQ41_07040) 1530812..1531042 - 231 WP_047292254.1 cysteine-rich CWC family protein -
GTQ41_RS07040 (GTQ41_07050) 1531415..1531699 + 285 WP_047292256.1 hypothetical protein -
GTQ41_RS07045 (GTQ41_07055) 1531879..1532277 + 399 WP_047292263.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6645.00 Da        Isoelectric Point: 11.2641

>T151475 WP_007963251.1 NZ_CP049737:c1528385-1528206 [Pseudomonas sp. AN-B15]
VNSRFLIGQLVADGWYLVRVKGSHHHFKHPIKPGLVTVPHPKKDLLKKTAISILKQALL

Download         Length: 180 bp

>T151475 NZ_CP065534:c1092054-1091851 [Lonsdalea populi]
ATGAAAAAAATAGATTACTTGATGCGTTTGCGCAAATGCACAACCATTGACACTCTTGAACGAGTTATTGAAAAAAACAA
GTATGAACTCTCAAACGATGAGTTAGAAATGTTCTATTCCGCGGCGGACCACCGTTTGGCCGAGCTGACCATGAATAAGC
TCTACGATAAAGTTCCCACCGCCGTATGGAAATACGTGCGTTAA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14493.47 Da        Isoelectric Point: 4.3833

>AT151475 WP_047292246.1 NZ_CP049737:c1528169-1527762 [Pseudomonas sp. AN-B15]
MLYPIAISIGDEEHAWGVEVPDIPGCFSAGDDLDDAVAMAREAIEGHFEILAEDGAPIPPASKLGVHVANPQYAGCAWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Download         Length: 408 bp

>AT151475 NZ_CP065534:c1092475-1092107 [Lonsdalea populi]
ATGGATGAGTACTCACCCAAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAGAATCTGCGCGATGAAAGTATCGC
GACTCTAGGTGACAGTAATCACGGCTGGGTGAATGATCCGACCTCCGCAATAAATCTTCAACTCAATGAACTTATTGAGC
ACATTGCGGCCTTTATTGGCACCTATAAAATTAAATATCCGGATGATGAAGAGTTGTACATGCGGGTTGAAAAATATCTA
GACGATACTTATATTCTTTTTAGCAATTACGGCATTAATGATGCAGAGCTGCGGAGATGGCAAAAATCTAAGTCGCAATT
ATTCAGAATGCTCTCAGAAAAGAGCAACTATGCAACAGTAAGAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W6VV48

Antitoxin

Source ID Structure
AlphaFold DB A0A2A2PH83

References