Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4642617..4643274 Replicon chromosome
Accession NZ_CP049290
Organism Enterobacter hormaechei subsp. steigerwaltii strain 08-175

Toxin (Protein)


Gene name cptA Uniprot ID A0A179PSF7
Locus tag G6324_RS22625 Protein ID WP_017382887.1
Coordinates 4642617..4643027 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID G8LDB7
Locus tag G6324_RS22630 Protein ID WP_003863437.1
Coordinates 4643008..4643274 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6324_RS22605 (G6324_22600) 4638615..4640348 - 1734 WP_023304383.1 single-stranded-DNA-specific exonuclease RecJ -
G6324_RS22610 (G6324_22605) 4640354..4641067 - 714 WP_023295380.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G6324_RS22615 (G6324_22610) 4641096..4641992 - 897 WP_023304385.1 site-specific tyrosine recombinase XerD -
G6324_RS22620 (G6324_22615) 4642094..4642615 + 522 WP_015571793.1 flavodoxin FldB -
G6324_RS22625 (G6324_22620) 4642617..4643027 - 411 WP_017382887.1 protein YgfX Toxin
G6324_RS22630 (G6324_22625) 4643008..4643274 - 267 WP_003863437.1 FAD assembly factor SdhE Antitoxin
G6324_RS22635 (G6324_22630) 4643569..4644549 + 981 WP_022649306.1 tRNA-modifying protein YgfZ -
G6324_RS22640 (G6324_22635) 4644661..4645320 - 660 WP_003863433.1 hemolysin III family protein -
G6324_RS22645 (G6324_22640) 4645587..4646318 + 732 WP_015571796.1 MurR/RpiR family transcriptional regulator -
G6324_RS22650 (G6324_22645) 4646435..4647868 + 1434 WP_017382891.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16321.21 Da        Isoelectric Point: 11.4775

>T151188 WP_017382887.1 NZ_CP049290:c4643027-4642617 [Enterobacter hormaechei subsp. steigerwaltii]
VVLWQSDLRVSWRSQWMSLLLHGLVAALVLLVPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWEMMGTPWMLNTGMMLRLRRVEDNRRQHLWLAADSMDPAEWRDLRRLIVQQPTQE

Download         Length: 411 bp

>T151188 NZ_CP065358:c5117511-5117125 [Kosakonia oryzae]
ATGGCTATTTATGTTTTAAAGGCGTTCGATAAGAACACCAAAGGCGATGGAATTGATGACGCAGCACTATGTAAAGCAGC
ACAGGAAGCCATTAGCGGAAAGTATGAGGCCAGCCTGGGTGGGGGTGTTTTTAAGAAGCGTATTCCACTTCCAGGCACTG
GGAAAAGTGGTGGCGCGCGAGCTGTTATCGCGTTCAAAATGGGAAAGCATTTATTCTTTTTAAACGGCTATGCCAAGTCT
GCCACTAGCAGTAATGGCAAAGAGATCCCGGATGATGTATTGAAGGCTTACCGTAAATTTGCTGCCGATTTATTCGGTAT
GACGCAAGCGCAGATAGATGCTCAAGTCCAGGCAGAAACGATGCGAGAGGTAAAATGCGATGGTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10526.96 Da        Isoelectric Point: 4.8310

>AT151188 WP_003863437.1 NZ_CP049290:c4643274-4643008 [Enterobacter hormaechei subsp. steigerwaltii]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
QERGPVAI

Download         Length: 267 bp

>AT151188 NZ_CP065358:c5117132-5116806 [Kosakonia oryzae]
ATGGTTGACCATCTGAAAGATTTGCAGGAAATGGCGCACGATATGTTTCCCCATGGTGGTATTTCTGAAGACACCGTTAA
CTACATTGACGCTCGCGTAAAACTGCGTGAGTTGCGTGCACGTTTGCCTGCTATCGTTGAAATGGATGGCGAGGCAATCC
GCGCCCTTCGTGGGCGCTATAACCTCAGCCAGGCTATGCTGGCTGAATATGTGAACATGTCAGTCGTGACCATTTCAAAA
TGGGAGAGAGGAGAAAAAAAACCGAACGGTGCAGCTCTCCGGGTGCTTAACACTCTTTCTGATAAAGGGCCGGAAATCTT
TATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A179PSF7

Antitoxin

Source ID Structure
AlphaFold DB A0A837F8P5

References