Detailed information of TA system TA151185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3596448..3597053
• Replicon: chromosome
• Accession: NZ_CP049290
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain 08-175

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TUP9
• Locus tag: G6324_RS17360
• Protein ID: WP_012906750.1
• Coordinates: 3596448..3596627 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A822X3T2
• Locus tag: G6324_RS17365
• Protein ID: WP_032619507.1
• Coordinates: 3596649..3597053 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6324_RS17335 (G6324_17335)	3591777..3592493	+	717	WP_032619512.1	ABC transporter ATP-binding protein	-
G6324_RS17340 (G6324_17340)	3592659..3593129	+	471	WP_017384432.1	hypothetical protein	-
G6324_RS17345 (G6324_17345)	3593135..3594061	-	927	WP_032619511.1	LysR substrate-binding domain-containing protein	-
G6324_RS17350 (G6324_17350)	3594091..3594414	-	324	WP_017384434.1	hypothetical protein	-
G6324_RS25085	3594417..3594764	-	348	WP_230199855.1	DUF4225 domain-containing protein	-
G6324_RS25090	3594831..3595172	-	342	WP_223862348.1	DUF4225 domain-containing protein	-
G6324_RS17360 (G6324_17360)	3596448..3596627	+	180	WP_012906750.1	type II toxin-antitoxin system HicA family toxin	Toxin
G6324_RS17365 (G6324_17365)	3596649..3597053	+	405	WP_032619507.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
G6324_RS17370 (G6324_17370)	3597094..3597762	-	669	WP_149350379.1	glycosyltransferase family 9 protein	-
G6324_RS17375 (G6324_17375)	3597778..3598164	-	387	WP_149350380.1	glycosyltransferase family 9 protein	-
G6324_RS17380 (G6324_17380)	3598241..3598819	-	579	WP_032619505.1	tail fiber assembly protein	-
G6324_RS17385 (G6324_17385)	3598819..3600996	-	2178	WP_058670599.1	phage tail protein	-
G6324_RS17390 (G6324_17390)	3600999..3601550	-	552	WP_032619504.1	phage tail protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fliA / gtrA / gtrB	3591789..3702033	110244

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6644.85 Da
• Isoelectric Point: 10.9132

>T151185 WP_012906750.1 NZ_CP049290:3596448-3596627 [Enterobacter hormaechei subsp. steigerwaltii]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Nucleotide

• Length: 180 bp

>T151185 NZ_CP065358:c4623362-4623150 [Kosakonia oryzae]
ATGAGACTGCGGCAATTATGTCGATGGTACTTTCTGGCAGCCCGCTTCCCGCGCAATATCGCGCAATATAACGATCATGC
ATTAACCGGCGATATGCTCGGTTTTCGCGAACTGCATATTGGCGGCGATTACTTACTGGTTTATCGCGTGGATGAAGACA
AGCATCTGGTGGTGTTTACCGATCTCGGGACACATGCTGAGCTTTTCGAATAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14676.53 Da
• Isoelectric Point: 4.2136

>AT151185 WP_032619507.1 NZ_CP049290:3596649-3597053 [Enterobacter hormaechei subsp. steigerwaltii]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGEDYQDAIESVREAIEAHIELLVEDGESVPEATTVENWLSDPEYAGAVWALVD
VDITRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVINQR

Nucleotide

• Length: 405 bp

>AT151185 NZ_CP065358:c4623724-4623452 [Kosakonia oryzae]
ATGGATTCTGTTATCCGATCGCGTATCGACAGCGAACTCAAAGCGCGAGCCGGGCTGGTGCTGGAAAGCTGCGGGCTTAA
CTGGAGTACGGCGATACGTCTGTTTGCTGAGCAAATTGTCAAAAACGAGGGTATCCCGTTTGAGGTCACCCGCAAACCCA
CGGCACGGTTACGCAACGCAATGAGCGAAGCCGAAGAGATCACCGCACACCAACATGGACGTTTTGAAGGTGTCGACCAG
CTAATGGATAGCTTAAACGATGGGAAAGAGTAA

Structures

Toxin

• AlphaFold DB: A0A7D6XDK4

Antitoxin

• AlphaFold DB: A0A822X3T2